Effect of air-drying on the generation of vitamin D2 and 25-hydroxyvitamin D2 by pulsed UV irradiation in button mushroom (Agaricus bisporus)

Glenn Cardwell, Janet F. Bornman, Anthony P. James, Alison Daly, Norbert Strobel, Jette Jakobsen, Lucinda J. Black*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Fresh and dried mushrooms naturally generate vitamin D2 when exposed to ultraviolet (UV) radiation. Vitamin D2 and 25-hydroxyvitamin D2 (25(OH)D2) concentrations were compared in dried button mushrooms (Agaricus bisporus) exposed to pulsed UV radiation either before or after air-drying. A further aim was to assess the effect of air-drying on the generation of D vitamers. Fresh button mushrooms were irradiated (Irr) with a total of 200 mJ/cm2 pulsed UV radiation before (Irr/AD) or after (AD/Irr) being air-dried (AD). A third group of fresh button mushrooms was irradiated but not air-dried (Irr). Control mushrooms were fresh and untreated. The D vitamers were quantified in freeze-dried samples using triple quadrupole mass spectrometry. Irr/AD mushrooms had more than double the concentration of vitamin D2 than AD/Irr mushrooms (9.5 µg/g dry weight (dw) vs 4.6 µg/g dw). However, Irr mushrooms contained 6.3 µg/g dw. The concentration of 25(OH)D2 in Irr mushrooms was 0.05 μg/g dw, while 0.14 μg/g dw was detected in Irr/AD mushrooms. There was no detectable 25(OH)D2 in control mushrooms, nor in AD/Irr mushrooms. The sequence of irradiating and drying mushrooms was a key factor in generating vitamin D2.
Original languageEnglish
Article number105034
JournalJournal of Food Composition and Analysis
Volume115
Number of pages6
ISSN0889-1575
DOIs
Publication statusPublished - 2023

Keywords

  • Agaricus bisporus
  • Mushroom
  • Vitamin D2
  • 25-hydroxyvitamin D2
  • Pulsed ultraviolet radiation
  • Air-drying

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