Ecology of uncultivated Oscillospira species in the rumen of cattle, sheep, and reindeer as assessed by microscopy and molecular approaches

R. I. Mackie, Rustam Aminov, W. P. Hu, A. V. Klieve, D. Ouwerkerk, M. A. Sundset, Y. Kamagata

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Abstract

The ecology of the uncultured, but large and morphologically conspicuous, rumen bacterium Oscillospira spp. was studied. Oscillospira-specific 16S rRNA gene sequences were detected in North American domestic cattle, sheep from Australia and Japan, and Norwegian reindeer. Phylogenetic analysis of the sequences obtained allowed definition of three operational taxonomic units within the Oscillospira clade. Consistent with this genetic diversity, we observed atypical smaller morphotypes by using an Oscillospira-specific fluorescence in situ hybridization probe. Despite the visual disappearance of typical large Oscillospira morphotypes, the presence of Oscillospira spp. was still detected by Oscillospira-specific PCR in the rumen of cattle and sheep. These observations suggest the broad presence of Oscillospira species in various rumen ecosystems with the level, and most likely the morphological form, dependent on diet. An ecological analysis based on enumeration of the morphologically conspicuous, large-septate form confirms that the highest counts are associated with the feeding of fresh forage diets to cattle and sheep and in two different subspecies of reindeer investigated.
Original languageEnglish
JournalAPPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume69
Issue number11
Pages (from-to)6808-6815
Number of pages8
ISSN0099-2240
DOIs
Publication statusPublished - 2003
Externally publishedYes

Keywords

  • BACTERIAL ecology
  • Animal Feed
  • Animals
  • Cattle
  • DNA, Bacterial
  • DNA, Ribosomal
  • Ecosystem
  • Electrophoresis
  • Female
  • Genes, rRNA
  • Genetic Variation
  • In Situ Hybridization, Fluorescence
  • Lactobacillaceae
  • Male
  • Polymerase Chain Reaction
  • RNA, Ribosomal, 16S
  • Reindeer
  • Rumen
  • Ruminants
  • Sheep
  • Bacteria
  • Biodiversity
  • Ecology
  • Fluorescence
  • Microbiology
  • RNA 16S
  • article
  • cattle
  • cell structure
  • ecology
  • electron microscopy
  • gene sequence
  • genetic variability
  • in situ hybridization
  • nonhuman
  • nucleotide sequence
  • oscillospira
  • phylogeny
  • reindeer
  • rumen microorganism
  • sequence analysis
  • sheep
  • taxonomy
  • Variation (Genetics)
  • Bacteria (microorganisms)
  • Bos taurus
  • Oscillospira
  • Ovis aries
  • Rangifer tarandus
  • Gene sequences
  • X
  • BIOTECHNOLOGY
  • MICROBIOLOGY
  • 16S RIBOSOMAL-RNA
  • IN-SITU DETECTION
  • PHYLOGENETIC ANALYSIS
  • SP-NOV
  • SEQUENCE-ANALYSIS
  • DIURNAL CHANGES
  • GEN-NOV
  • ORGANISMS
  • BACTERIA
  • GUILLERMONDII
  • Artiodactyla Mammalia Vertebrata Chordata Animalia (Animals, Artiodactyls, Chordates, Mammals, Nonhuman Vertebrates, Nonhuman Mammals, Vertebrates) - Bovidae [85715] cattle common sheep common
  • Artiodactyla Mammalia Vertebrata Chordata Animalia (Animals, Artiodactyls, Chordates, Mammals, Nonhuman Vertebrates, Nonhuman Mammals, Vertebrates) - Cervidae [85725] reindeer common
  • Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Endospore-forming Gram-Positives [07810] Oscillospira spp. species
  • Oscillospira spp. 16S rRNA gene [Endospore-forming Gram-Positives] Oscillospira spp. 16S ribosomal RNA gene
  • 03502, Genetics - General
  • 03506, Genetics - Animal
  • 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
  • 14004, Digestive system - Physiology and biochemistry
  • 31000, Physiology and biochemistry of bacteria
  • 31500, Genetics of bacteria and viruses
  • Biochemistry and Molecular Biophysics
  • Ingestion and Assimilation
  • rumen digestive system
  • denaturing gradient gel electrophoresis electrophoretic techniques, laboratory techniques
  • fluorescence in situ hybridization genetic techniques, laboratory techniques
  • microscopy imaging and microscopy techniques, laboratory techniques
  • PCR polymerase chain reaction genetic techniques, laboratory techniques
  • phylogenetic analysis mathematical and computer techniques
  • Digestive System
  • Methods and Techniques
  • Molecular Genetics

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