Ebolavirus comparative genomics

Se-Ran Jun, Michael R. Leuze, Intawat Nookaew, Edward C. Uberbacher, Miriam Land, Qian Zhang, Visanu Wanchai, Juanjuan Chai, Morten Nielsen, Thomas Trolle, Ole Lund, Gregory S. Buzard, Thomas Dybdal Pedersen, Trudy M. Wassenaar, David W. Ussery

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    Abstract

    The 2014 Ebola outbreak in West Africa is the largest documented for this virus. To examine the dynamics of this genome, we compare more than 100 currently available ebolavirus genomes to each other and to other viral genomes. Based on oligomer frequency analysis, the family Filoviridae forms a distinct group from all other sequenced viral genomes. All filovirus genomes sequenced to date encode proteins with similar functions and gene order, although there is considerable divergence in sequences between the three genera Ebolavirus, Cuevavirus and Marburgvirus within the family Filoviridae. Whereas all ebolavirus genomes are quite similar (multiple sequences of the same strain are often identical), variation is most common in the intergenic regions and within specific areas of the genes encoding the glycoprotein (GP), nucleoprotein (NP) and polymerase (L). We predict regions that could contain epitope-binding sites, which might be good vaccine targets. This information, combined with glycosylation sites and experimentally determined epitopes, can identify the most promising regions for the development of therapeutic strategies.


    This manuscript has been authored by UT-Battelle, LLC under Contract No. DE-AC05-00OR22725 with the U.S. Department of Energy. The United States Government retains and the publisher, by accepting the article for publication, acknowledges that the United States Government retains a non-exclusive, paid-up, irrevocable, world-wide license to publish or reproduce the published form of this manuscript, or allow others to do so, for United States Government purposes. The Department of Energy will provide public access to these results of federally sponsored research in accordance with the DOE Public Access Plan (http://energy.gov/downloads/doe-public-access-plan).
    Original languageEnglish
    Article numberfuv031
    JournalF E M S Microbiology Reviews
    Volume479
    Issue number1
    Pages (from-to)764-778
    Number of pages15
    ISSN0168-6445
    DOIs
    Publication statusPublished - 2015

    Bibliographical note

    This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

    Keywords

    • Ebola
    • Comparative genomics
    • Viral genomes
    • Epitope prediction
    • Ebola virus disease (EVD)
    • Filovirus

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