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Abstract
Saccharomyces cerevisiae is one of the key cell factories for production of chemicals and active pharmaceuticals. For large-scale fermentations, particularly in biorefinery applications, it is desirable to use stress-tolerant industrial strains. However, such strains are less amenable for metabolic engineering than the standard laboratory strains. To enable easy delivery and overexpression of genes in a wide range of industrial S. cerevisiae strains, we constructed a set of integrative vectors with long homology arms and dominant selection markers. The vectors integrate into previously validated chromosomal locations via double cross-over and result in homogenous stable expression of the integrated genes, as shown for several unrelated industrial strains. Cre-mediated marker rescue is possible for removing markers positioned on different chromosomes. To demonstrate the applicability of the presented vector set for metabolic engineering of industrial yeast, we constructed xylose-utilizing strains overexpressing xylose isomerase, xylose transporter and five genes of the pentose phosphate pathway.
Original language | English |
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Journal | Journal of Industrial Microbiology and Biotechnology |
Volume | 42 |
Issue number | 11 |
Pages (from-to) | 1519-1531 |
Number of pages | 13 |
ISSN | 1367-5435 |
DOIs | |
Publication status | Published - Oct 2015 |
Bibliographical note
This article is published with open access at Springerlink.comKeywords
- Industrial yeast
- Integrative vectors
- Heterologous gene expression
- Metabolic engineering
- Xylose utilization
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Dive into the research topics of 'EasyClone 2.0: expanded toolkit of integrative vectors for stable gene expression in industrial Saccharomyces cerevisiae strains'. Together they form a unique fingerprint.Projects
- 1 Finished
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BioREFINE-2G: Development of 2nd Generation Biorefineries Production of Dicarboxylic Acids and Bio-based Polymers Derived Thereof
Förster, J. (Project Coordinator), Borodina, I. (Project Coordinator), Stovicek, V. (Project Participant), Ögmundarson, Ó. (PhD Student), Rasmussen, B. K. (Project Participant), Lis, A. V. (Project Participant) & Lohmann, R. (Project Participant)
European Commission- European Research Area
01/10/2013 → 30/09/2017
Project: Research