In total, 56 matched pT1 CRCs were thoroughly clinico-pathologically characterized and miRNA microarrays were performed on invasive front tissue samples. Global miRNA intensities were screened using paired t-tests between pT1pN+ and pT1pN0. Associations between miR-17/92 and histopathological features were analyzed using general linear models and tumor cell adjusted expression intensities.
miR-17-3p and miR-92a were significantly higher expressed in the invasive front of tumors with LNM compared to those without, corresponding to 1.53 fold higher expression of miR-17-3p (95%CI: 1.04–2.24, P = .030) and 1.28 fold higher expression of miR-92a (95%CI: 1.01–1.68, P = .042). An inverse association between miR-19a and presence of high grade tumor budding was observed (1.55 fold, 95%CI: 1.13–2.12, P = .008). We provide evidence for associations between early regional LNM and high expression levels of the miR-17/92 cluster members: miR-17-3p and miR-92a, in the invasive front of CRC. Our results support a role for the miR-17/92 cluster in early metastatic progression of CRC and calls for further investigation.
- Colorectal Cancer
- Invasive Tumor Front