Dynamics of biofilm formation in a model drinking water distribution system

Rasmus Boe-Hansen, Hans-Jørgen Albrechtsen, Erik Arvin, C. Jorgensen

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The dynamics of biofilm formation in non-chlorinated groundwater-based drinking water was studied in a model distribution system. The formation of biofilm was closely monitored for a period of 522 days by total bacterial counts (AODC), heterotrophic plate counts (R2A media), and ATP content determinations. The biofilm grew at a rate of 0.030±0.002 day−1 reaching quasi-stationary state at 2.6×106 cells/cm2 after approximately 200 days. The low substrate level in the bulk phase (AOC at approximately 6 g ac-C/l) most likely caused the relatively slow biofilm formation rate observed. During the maturation of the biofilm, the bacterial community changed properties in terms of cell-specific ATP content and culturability of the bacteria.
Original languageEnglish
JournalJournal of Water Supply: Research and Technology. AQUA
Pages (from-to)399-406
Publication statusPublished - 2002


  • stationary phase
  • biofilm
  • drinking water
  • growth
  • formation

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