Abstract
| Original language | English |
|---|---|
| Journal | BIOPHYSICAL JOURNAL |
| Volume | 106 |
| Issue number | 9 |
| Pages (from-to) | 1864-1870 |
| Number of pages | 7 |
| ISSN | 0006-3495 |
| DOIs | |
| Publication status | Published - 2014 |
| Externally published | Yes |
Bibliographical note
© 2014 by the Biophysical SocietyCite this
}
Dynamic Mechanical Responses of Arabidopsis Thylakoid Membranes during PSII-Specific Illumination. / Clausen, Casper Hyttel; Brooks, Matthew D.; Li, Tai-De; Grob, Patricia; Kemalyan, Gigi; Nogales, Eva; Niyogi, Krishna K.; Fletcher, Daniel A.
In: BIOPHYSICAL JOURNAL, Vol. 106, No. 9, 2014, p. 1864-1870.Research output: Contribution to journal › Journal article › Research › peer-review
TY - JOUR
T1 - Dynamic Mechanical Responses of Arabidopsis Thylakoid Membranes during PSII-Specific Illumination
AU - Clausen, Casper Hyttel
AU - Brooks, Matthew D.
AU - Li, Tai-De
AU - Grob, Patricia
AU - Kemalyan, Gigi
AU - Nogales, Eva
AU - Niyogi, Krishna K.
AU - Fletcher, Daniel A.
N1 - © 2014 by the Biophysical Society
PY - 2014
Y1 - 2014
N2 - Remodeling of thylakoid membranes in response to illumination is an important process for the regulation of photosynthesis. We investigated the thylakoid network from Arabidopsis thaliana using atomic force microscopy to capture dynamic changes in height, elasticity, and viscosity of isolated thylakoid membranes caused by changes in illumination. We also correlated the mechanical response of the thylakoid network with membrane ultrastructure using electron microscopy. We find that the elasticity of the thylakoid membranes increases immediately upon PSII-specific illumination, followed by a delayed height change. Direct visualization by electron microscopy confirms that there is a significant change in the packing repeat distance of the membrane stacks in response to illumination. Although experiments with Gramicidin show that the change in elasticity depends primarily on the transmembrane pH gradient, the height change requires both the pH gradient and STN7-kinase-dependent phosphorylation of LHCII. Our studies indicate that lumen expansion in response to illumination is not simply a result of the influx of water, and we propose a dynamic model in which protein interactions within the lumen drive these changes.
AB - Remodeling of thylakoid membranes in response to illumination is an important process for the regulation of photosynthesis. We investigated the thylakoid network from Arabidopsis thaliana using atomic force microscopy to capture dynamic changes in height, elasticity, and viscosity of isolated thylakoid membranes caused by changes in illumination. We also correlated the mechanical response of the thylakoid network with membrane ultrastructure using electron microscopy. We find that the elasticity of the thylakoid membranes increases immediately upon PSII-specific illumination, followed by a delayed height change. Direct visualization by electron microscopy confirms that there is a significant change in the packing repeat distance of the membrane stacks in response to illumination. Although experiments with Gramicidin show that the change in elasticity depends primarily on the transmembrane pH gradient, the height change requires both the pH gradient and STN7-kinase-dependent phosphorylation of LHCII. Our studies indicate that lumen expansion in response to illumination is not simply a result of the influx of water, and we propose a dynamic model in which protein interactions within the lumen drive these changes.
U2 - 10.1016/j.bpj.2014.03.016
DO - 10.1016/j.bpj.2014.03.016
M3 - Journal article
C2 - 24806918
VL - 106
SP - 1864
EP - 1870
JO - Biophysical Journal
JF - Biophysical Journal
SN - 0006-3495
IS - 9
ER -