Dynamic Mechanical Responses of Arabidopsis Thylakoid Membranes during PSII-Specific Illumination

Casper Hyttel Clausen, Matthew D. Brooks, Tai-De Li, Patricia Grob, Gigi Kemalyan, Eva Nogales, Krishna K. Niyogi, Daniel A. Fletcher

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Abstract

Remodeling of thylakoid membranes in response to illumination is an important process for the regulation of photosynthesis. We investigated the thylakoid network from Arabidopsis thaliana using atomic force microscopy to capture dynamic changes in height, elasticity, and viscosity of isolated thylakoid membranes caused by changes in illumination. We also correlated the mechanical response of the thylakoid network with membrane ultrastructure using electron microscopy. We find that the elasticity of the thylakoid membranes increases immediately upon PSII-specific illumination, followed by a delayed height change. Direct visualization by electron microscopy confirms that there is a significant change in the packing repeat distance of the membrane stacks in response to illumination. Although experiments with Gramicidin show that the change in elasticity depends primarily on the transmembrane pH gradient, the height change requires both the pH gradient and STN7-kinase-dependent phosphorylation of LHCII. Our studies indicate that lumen expansion in response to illumination is not simply a result of the influx of water, and we propose a dynamic model in which protein interactions within the lumen drive these changes.
Original languageEnglish
JournalBIOPHYSICAL JOURNAL
Volume106
Issue number9
Pages (from-to)1864-1870
Number of pages7
ISSN0006-3495
DOIs
Publication statusPublished - 2014
Externally publishedYes

Bibliographical note

© 2014 by the Biophysical Society

Cite this

Clausen, C. H., Brooks, M. D., Li, T-D., Grob, P., Kemalyan, G., Nogales, E., ... Fletcher, D. A. (2014). Dynamic Mechanical Responses of Arabidopsis Thylakoid Membranes during PSII-Specific Illumination. BIOPHYSICAL JOURNAL, 106(9), 1864-1870. https://doi.org/10.1016/j.bpj.2014.03.016
Clausen, Casper Hyttel ; Brooks, Matthew D. ; Li, Tai-De ; Grob, Patricia ; Kemalyan, Gigi ; Nogales, Eva ; Niyogi, Krishna K. ; Fletcher, Daniel A. / Dynamic Mechanical Responses of Arabidopsis Thylakoid Membranes during PSII-Specific Illumination. In: BIOPHYSICAL JOURNAL. 2014 ; Vol. 106, No. 9. pp. 1864-1870.
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title = "Dynamic Mechanical Responses of Arabidopsis Thylakoid Membranes during PSII-Specific Illumination",
abstract = "Remodeling of thylakoid membranes in response to illumination is an important process for the regulation of photosynthesis. We investigated the thylakoid network from Arabidopsis thaliana using atomic force microscopy to capture dynamic changes in height, elasticity, and viscosity of isolated thylakoid membranes caused by changes in illumination. We also correlated the mechanical response of the thylakoid network with membrane ultrastructure using electron microscopy. We find that the elasticity of the thylakoid membranes increases immediately upon PSII-specific illumination, followed by a delayed height change. Direct visualization by electron microscopy confirms that there is a significant change in the packing repeat distance of the membrane stacks in response to illumination. Although experiments with Gramicidin show that the change in elasticity depends primarily on the transmembrane pH gradient, the height change requires both the pH gradient and STN7-kinase-dependent phosphorylation of LHCII. Our studies indicate that lumen expansion in response to illumination is not simply a result of the influx of water, and we propose a dynamic model in which protein interactions within the lumen drive these changes.",
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Clausen, CH, Brooks, MD, Li, T-D, Grob, P, Kemalyan, G, Nogales, E, Niyogi, KK & Fletcher, DA 2014, 'Dynamic Mechanical Responses of Arabidopsis Thylakoid Membranes during PSII-Specific Illumination', BIOPHYSICAL JOURNAL, vol. 106, no. 9, pp. 1864-1870. https://doi.org/10.1016/j.bpj.2014.03.016

Dynamic Mechanical Responses of Arabidopsis Thylakoid Membranes during PSII-Specific Illumination. / Clausen, Casper Hyttel; Brooks, Matthew D.; Li, Tai-De; Grob, Patricia; Kemalyan, Gigi; Nogales, Eva; Niyogi, Krishna K.; Fletcher, Daniel A.

In: BIOPHYSICAL JOURNAL, Vol. 106, No. 9, 2014, p. 1864-1870.

Research output: Contribution to journalJournal articleResearchpeer-review

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T1 - Dynamic Mechanical Responses of Arabidopsis Thylakoid Membranes during PSII-Specific Illumination

AU - Clausen, Casper Hyttel

AU - Brooks, Matthew D.

AU - Li, Tai-De

AU - Grob, Patricia

AU - Kemalyan, Gigi

AU - Nogales, Eva

AU - Niyogi, Krishna K.

AU - Fletcher, Daniel A.

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AB - Remodeling of thylakoid membranes in response to illumination is an important process for the regulation of photosynthesis. We investigated the thylakoid network from Arabidopsis thaliana using atomic force microscopy to capture dynamic changes in height, elasticity, and viscosity of isolated thylakoid membranes caused by changes in illumination. We also correlated the mechanical response of the thylakoid network with membrane ultrastructure using electron microscopy. We find that the elasticity of the thylakoid membranes increases immediately upon PSII-specific illumination, followed by a delayed height change. Direct visualization by electron microscopy confirms that there is a significant change in the packing repeat distance of the membrane stacks in response to illumination. Although experiments with Gramicidin show that the change in elasticity depends primarily on the transmembrane pH gradient, the height change requires both the pH gradient and STN7-kinase-dependent phosphorylation of LHCII. Our studies indicate that lumen expansion in response to illumination is not simply a result of the influx of water, and we propose a dynamic model in which protein interactions within the lumen drive these changes.

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JO - Biophysical Journal

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