Dynamic investigation and modeling of the nitrogen co-metabolism in Methylococcus capsulatus (Bath)

Research output: Contribution to journalJournal article – Annual report year: 2019Researchpeer-review

DOI

View graph of relations

The methanotrophic bacterium Methylococcus capsulatus is capable of assimilating methane and oxygen into a protein rich biomass, however the diverse metabolism of the microorganism also allows for several undesired co-metabolic side-reactions to occur. In this study, the ammonia co-metabolism in Methylococcus capsulatus is investigated using pulse experiments. Surprisingly Methylococcus capsulatus oxidizes ammonia to nitrate through a yet unknown mechanism, and fixes molecular nitrogen even at a high dissolved oxygen tension. The observed phenomena can be modeled using 14 ordinary differential equations and 18 kinetic parameters, of which 6 were revealed by Morris screening to be identifiable from the experimental data. Monte Carlo simulations showed that the model was robust and accurate even with uncertainty in the parameter values as confirmed by a statistical error analysis.
Original languageEnglish
JournalBiotechnology and Bioengineering
ISSN0006-3592
DOIs
Publication statusAccepted/In press - 2019
CitationsWeb of Science® Times Cited: No match on DOI

    Research areas

  • Dynamic Model, Methanotrophs, Methylococcus, Nitrogen metabolism, Pulse experiment, SCP, Sensitivity, Uncertainty
Download as:
Download as PDF
Select render style:
APAAuthorCBE/CSEHarvardMLAStandardVancouverShortLong
PDF
Download as HTML
Select render style:
APAAuthorCBE/CSEHarvardMLAStandardVancouverShortLong
HTML
Download as Word
Select render style:
APAAuthorCBE/CSEHarvardMLAStandardVancouverShortLong
Word

ID: 185414594