Dynamic investigation and modeling of the nitrogen co-metabolism in Methylococcus capsulatus (Bath)

Leander A. H. Petersen, Christian Lieven, Subir K. Nandy, John Villadsen, Sten B. Jørgensen, Ib Christensen, Krist V. Gernaey*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The methanotrophic bacterium Methylococcus capsulatus is capable of assimilating methane and oxygen into a protein rich biomass, however the diverse metabolism of the microorganism also allows for several undesired co-metabolic side-reactions to occur. In this study, the ammonia co-metabolism in Methylococcus capsulatus is investigated using pulse experiments. Surprisingly Methylococcus capsulatus oxidizes ammonia to nitrate through a yet unknown mechanism, and fixes molecular nitrogen even at a high dissolved oxygen tension. The observed phenomena can be modeled using 14 ordinary differential equations and 18 kinetic parameters, of which 6 were revealed by Morris screening to be identifiable from the experimental data. Monte Carlo simulations showed that the model was robust and accurate even with uncertainty in the parameter values as confirmed by a statistical error analysis.
Original languageEnglish
JournalBiotechnology and Bioengineering
Volume116
Issue number11
Pages (from-to)2884-2895
ISSN0006-3592
DOIs
Publication statusPublished - 2019

Keywords

  • Dynamic Model
  • Methanotrophs
  • Methylococcus
  • Nitrogen metabolism
  • Pulse experiment
  • SCP
  • Sensitivity
  • Uncertainty

Cite this

@article{fbe1652380024a9ba9595f9281e98933,
title = "Dynamic investigation and modeling of the nitrogen co-metabolism in Methylococcus capsulatus (Bath)",
abstract = "The methanotrophic bacterium Methylococcus capsulatus is capable of assimilating methane and oxygen into a protein rich biomass, however the diverse metabolism of the microorganism also allows for several undesired co-metabolic side-reactions to occur. In this study, the ammonia co-metabolism in Methylococcus capsulatus is investigated using pulse experiments. Surprisingly Methylococcus capsulatus oxidizes ammonia to nitrate through a yet unknown mechanism, and fixes molecular nitrogen even at a high dissolved oxygen tension. The observed phenomena can be modeled using 14 ordinary differential equations and 18 kinetic parameters, of which 6 were revealed by Morris screening to be identifiable from the experimental data. Monte Carlo simulations showed that the model was robust and accurate even with uncertainty in the parameter values as confirmed by a statistical error analysis.",
keywords = "Dynamic Model, Methanotrophs, Methylococcus, Nitrogen metabolism, Pulse experiment, SCP, Sensitivity, Uncertainty",
author = "Petersen, {Leander A. H.} and Christian Lieven and Nandy, {Subir K.} and John Villadsen and J{\o}rgensen, {Sten B.} and Ib Christensen and Gernaey, {Krist V.}",
year = "2019",
doi = "10.1002/bit.27113",
language = "English",
volume = "116",
pages = "2884--2895",
journal = "Biotechnology and Bioengineering (Print)",
issn = "0006-3592",
publisher = "JohnWiley & Sons, Inc.",
number = "11",

}

Dynamic investigation and modeling of the nitrogen co-metabolism in Methylococcus capsulatus (Bath). / Petersen, Leander A. H.; Lieven, Christian; Nandy, Subir K.; Villadsen, John; Jørgensen, Sten B.; Christensen, Ib; Gernaey, Krist V.

In: Biotechnology and Bioengineering, Vol. 116, No. 11, 2019, p. 2884-2895.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Dynamic investigation and modeling of the nitrogen co-metabolism in Methylococcus capsulatus (Bath)

AU - Petersen, Leander A. H.

AU - Lieven, Christian

AU - Nandy, Subir K.

AU - Villadsen, John

AU - Jørgensen, Sten B.

AU - Christensen, Ib

AU - Gernaey, Krist V.

PY - 2019

Y1 - 2019

N2 - The methanotrophic bacterium Methylococcus capsulatus is capable of assimilating methane and oxygen into a protein rich biomass, however the diverse metabolism of the microorganism also allows for several undesired co-metabolic side-reactions to occur. In this study, the ammonia co-metabolism in Methylococcus capsulatus is investigated using pulse experiments. Surprisingly Methylococcus capsulatus oxidizes ammonia to nitrate through a yet unknown mechanism, and fixes molecular nitrogen even at a high dissolved oxygen tension. The observed phenomena can be modeled using 14 ordinary differential equations and 18 kinetic parameters, of which 6 were revealed by Morris screening to be identifiable from the experimental data. Monte Carlo simulations showed that the model was robust and accurate even with uncertainty in the parameter values as confirmed by a statistical error analysis.

AB - The methanotrophic bacterium Methylococcus capsulatus is capable of assimilating methane and oxygen into a protein rich biomass, however the diverse metabolism of the microorganism also allows for several undesired co-metabolic side-reactions to occur. In this study, the ammonia co-metabolism in Methylococcus capsulatus is investigated using pulse experiments. Surprisingly Methylococcus capsulatus oxidizes ammonia to nitrate through a yet unknown mechanism, and fixes molecular nitrogen even at a high dissolved oxygen tension. The observed phenomena can be modeled using 14 ordinary differential equations and 18 kinetic parameters, of which 6 were revealed by Morris screening to be identifiable from the experimental data. Monte Carlo simulations showed that the model was robust and accurate even with uncertainty in the parameter values as confirmed by a statistical error analysis.

KW - Dynamic Model

KW - Methanotrophs

KW - Methylococcus

KW - Nitrogen metabolism

KW - Pulse experiment

KW - SCP

KW - Sensitivity

KW - Uncertainty

U2 - 10.1002/bit.27113

DO - 10.1002/bit.27113

M3 - Journal article

VL - 116

SP - 2884

EP - 2895

JO - Biotechnology and Bioengineering (Print)

JF - Biotechnology and Bioengineering (Print)

SN - 0006-3592

IS - 11

ER -