dye (arc) mutants: insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in Escherichia coli recombinants

Jimena A. Ruiz, Ruben O. Fernandez, Pablo Ivan Nikel, Beatriz S. Méndez, M. Julia Pettinari

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

arcA codes for a central regulator in Escherichia coli that responds to redox conditions of growth. Mutations in this gene, originally named dye, confer sensitivity to toluidine blue and other redox dyes. However, the molecular basis for the dye-sensitive phenotype has not been elucidated. In this work, we show that toluidine blue redirects electrons to O2 and causes an increase in the generation of reactive O2 species (ROS). We also demonstrate that synthesis of poly (3-hydroxybutyrate) suppresses the Dye phenotype in E. coli recombinants, as the capacity to synthesize the polymer reduces sensitivity to toluidine blue, O2 consumption and ROS production levels.
Original languageEnglish
JournalFEMS Microbiology Letters
Volume258
Issue number1
Pages (from-to)55-60
Number of pages6
ISSN0378-1097
DOIs
Publication statusPublished - 2006
Externally publishedYes

Keywords

  • arcA
  • O2 consumption
  • PHB
  • ROS
  • Toluidine blue

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