Dual initiation sites of protein-synthesis on foot-and-mouth-disease virus-RNA are selected following internal entry and scanning of ribosomes invivo

Graham Belsham

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The initiation of protein synthesis on foot-and-mouth disease virus RNA occurs at two sites separated by 84 nucleotides. Immediately upstream from the first of these sites is the internal ribosome entry site (IRES), which directs the translation of this RNA to be cap-independent. The utilization of these two initiation sites has been examined using artificial fusion genes in vivo under a variety of conditions. Additional in-frame AUG codons have been introduced between these two authentic start sites to determine the mechanism by which ribosomes recognize the second start site. The results indicate that following internal entry of ribosomes on the 5' side of the first initiation codon, many fail to initiate protein synthesis at this position and scan along the RNA to the second initiation site. In the presence or absence of the IRES both initiation sites are efficiently used but the utilization of the two sites is slightly biased towards the second initiation site by the IRES. Furthermore, in the presence of the IRES, protein synthesis initiates at both sites independently of the activity of the cap-binding complex.
Original languageEnglish
JournalE M B O Journal
Volume11
Issue number3
Pages (from-to)1105-1110
ISSN0261-4189
Publication statusPublished - 1992
Externally publishedYes

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