Dual DNA vaccination of rainbow trout (Oncorhynchus mykiss) against two different rhabdoviruses, VHSV and IHNV, induces specific divalent protection

Katja Einer-Jensen, L. Delgado, Ellen Lorenzen, G. Bovo, Ø. Evensen, S. LaPatra, Niels Lorenzen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    DNA vaccines encoding the glycoprotein genes of the salmonid rhabdoviruses VHSV and IHNV are very efficient in eliciting protective immune responses against their respective diseases in rainbow trout (Oncorhynchus mykiss). The early anti-viral response (EAVR) provides Protection by 4 days post vaccination and is non-specific and transient while the specific anti-viral response (SAVR) is long lasting and highly specific. Since both VHSV and IHNV are endemic in rainbow trout in several geographical regions of Europe and Atlantic salmon (Salmo salar) on the Pacific coast of North America, co-vaccination against the two diseases would be a preferable option. In the present study we demonstrated that a single injection of mixed DNA vaccines induced long-lasting protection against both individual and a simultaneous virus challenge 80 days post vaccination. Transfected muscle cells at the injection site expressed both G proteins. This study confirms the applied potential Of using a combined DNA vaccination for protection of fish against two different rhabdoviral diseases.
    Original languageEnglish
    JournalVaccine
    Volume27
    Issue number8
    Pages (from-to)1248-1253
    ISSN0264-410X
    DOIs
    Publication statusPublished - 2009

    Keywords

    • Immunology of protection
    • Disease prevention
    • Animal model

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