Drug quantification in turbid media by fluorescence imaging combined with light-absorption correction using white Monte Carlo simulations

Haiyan Xie, Haichun Liu, Pontus Svenmarker, Johan Axelsson, Can T. Xu, Susanna Gräfe, Jesper Holm Lundeman, Haynes Pak Hay Cheng, Sune Svanberg, Niels Bendsoe, Peter E. Andersen, Katarina Svanberg, Stefan Andersson-Engels

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Accurate quantification of photosensitizers is in many cases a critical issue in photodynamic therapy. As a noninvasive and sensitive tool, fluorescence imaging has attracted particular interest for quantification in pre-clinical research. However, due to the absorption of excitation and emission light by turbid media, such as biological tissue, the detected fluorescence signal does not have a simple and unique dependence on the fluorophore concentration for different tissues, but depends in a complex way on other parameters as well. For this reason, little has been done on drug quantification in vivo by the fluorescence imaging technique. In this paper we present a novel approach to compensate for the light absorption in homogeneous turbid media both for the excitation and emission light, utilizing time-resolved fluorescence white Monte Carlo simulations combined with the Beer-Lambert law. This method shows that the corrected fluorescence intensity is almost proportional to the absolute fluorophore concentration. The results on controllable tissue phantoms and murine tissues are presented and show good correlations between the evaluated fluorescence intensities after the light-absorption correction and absolute fluorophore concentrations. These results suggest that the technique potentially provides the means to quantify the fluorophore concentration from fluorescence images. © 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).
    Original languageEnglish
    JournalJournal of Biomedical Optics
    Volume16
    Issue number6
    Pages (from-to)066002
    ISSN1083-3668
    DOIs
    Publication statusPublished - 2011

    Keywords

    • Tissues
    • Fluorescence
    • Photon migration
    • Optical properties
    • Absorption
    • Imaging
    • Biomedical optics
    • Monte Carlo

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