Droplet-based microfluidics as a future tool for strain improvement in lactic acid bacteria

Jun Chen*, Mike Vestergaard, Jing Shen, Christian Solem, Martin Dufva, Peter Ruhdal Jensen

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Strain development is frequently used to improve the performance and functionality of industrially important microbes. As traditional mutagenesis screen is especially utilized by the food industry to improve strains used in food fermentation, high-throughput and cost-effective screening tools are important in mutant selection. The emerging droplet-based microfluidics technology miniaturizes the volume for cell cultivation and phenotype interrogation down to the pico-liter scales, which facilitates screening of microbes for improved phenotypical properties tremendously. In this mini-review, we present recent application of the droplet-based microfluidics in microbial strain improvement with a focus on its potential use in the screening of lactic acid bacteria.
Original languageEnglish
Article numberfny258
JournalFEMS Microbiology Letters
Volume23
Issue number1
ISSN0378-1097
DOIs
Publication statusPublished - 2018

Keywords

  • Lactic acid bacteria
  • Strain improvement
  • Droplet-based microfluidics
  • High-throughput screening
  • Starter culture
  • Classical mutagenesis

Cite this

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title = "Droplet-based microfluidics as a future tool for strain improvement in lactic acid bacteria",
abstract = "Strain development is frequently used to improve the performance and functionality of industrially important microbes. As traditional mutagenesis screen is especially utilized by the food industry to improve strains used in food fermentation, high-throughput and cost-effective screening tools are important in mutant selection. The emerging droplet-based microfluidics technology miniaturizes the volume for cell cultivation and phenotype interrogation down to the pico-liter scales, which facilitates screening of microbes for improved phenotypical properties tremendously. In this mini-review, we present recent application of the droplet-based microfluidics in microbial strain improvement with a focus on its potential use in the screening of lactic acid bacteria.",
keywords = "Lactic acid bacteria, Strain improvement, Droplet-based microfluidics, High-throughput screening, Starter culture, Classical mutagenesis",
author = "Jun Chen and Mike Vestergaard and Jing Shen and Christian Solem and Martin Dufva and Jensen, {Peter Ruhdal}",
year = "2018",
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Droplet-based microfluidics as a future tool for strain improvement in lactic acid bacteria. / Chen, Jun; Vestergaard, Mike; Shen, Jing; Solem, Christian; Dufva, Martin; Jensen, Peter Ruhdal.

In: FEMS Microbiology Letters, Vol. 23, No. 1, fny258, 2018.

Research output: Contribution to journalJournal articleResearchpeer-review

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T1 - Droplet-based microfluidics as a future tool for strain improvement in lactic acid bacteria

AU - Chen, Jun

AU - Vestergaard, Mike

AU - Shen, Jing

AU - Solem, Christian

AU - Dufva, Martin

AU - Jensen, Peter Ruhdal

PY - 2018

Y1 - 2018

N2 - Strain development is frequently used to improve the performance and functionality of industrially important microbes. As traditional mutagenesis screen is especially utilized by the food industry to improve strains used in food fermentation, high-throughput and cost-effective screening tools are important in mutant selection. The emerging droplet-based microfluidics technology miniaturizes the volume for cell cultivation and phenotype interrogation down to the pico-liter scales, which facilitates screening of microbes for improved phenotypical properties tremendously. In this mini-review, we present recent application of the droplet-based microfluidics in microbial strain improvement with a focus on its potential use in the screening of lactic acid bacteria.

AB - Strain development is frequently used to improve the performance and functionality of industrially important microbes. As traditional mutagenesis screen is especially utilized by the food industry to improve strains used in food fermentation, high-throughput and cost-effective screening tools are important in mutant selection. The emerging droplet-based microfluidics technology miniaturizes the volume for cell cultivation and phenotype interrogation down to the pico-liter scales, which facilitates screening of microbes for improved phenotypical properties tremendously. In this mini-review, we present recent application of the droplet-based microfluidics in microbial strain improvement with a focus on its potential use in the screening of lactic acid bacteria.

KW - Lactic acid bacteria

KW - Strain improvement

KW - Droplet-based microfluidics

KW - High-throughput screening

KW - Starter culture

KW - Classical mutagenesis

U2 - 10.1093/femsle/fny258

DO - 10.1093/femsle/fny258

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JO - F E M S Microbiology Letters

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