Domestication Of Pantoea Sp. Through Genome-Scale Metabolic Modelling And A Genetic Toolbox

Lies van der Heijden*, Lucas Nicolaas Ludovic van der Maas, Philip Johannes Gorter de Vries, Emre Özdemir, Sheila Ingemann Jensen

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

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Abstract

The genus Pantoea comprises a versatile group of Gram-negative bacteria belonging to the family of Erwiniaceae. The species in this genus have been isolated from several environments, such as water, soil, plants and animals. The ubiquity and versatility of Pantoea isolates make it an ideal genus for exploring niche-specific adaptation and opportunism, as well as for developing commercially relevant therapeutic, agricultural and environmental products.

Our fundamental understanding of Pantoea, however, remains limited due to the absence of a standardized genetic toolbox for this genus. Additionally, only approximately 30 genome-sequenced strains are available to date and no genome-scale metabolic model is available. 
In this study, a Pantoea strain was isolated from soil samples and characterized as a newly discovered strain; Pantoea sp.. To further develop Pantoea strains into fully optimized microbial chassis, an in-depth, systems-level understanding of metabolism is required, for which genome-scale metabolic models are essential. We have developed a draft genome-scale metabolic model using CarveMe (Machado et al., 2018). The model is curated using additional data derived from wet-lab
experiments.

Additionally, Synthetic biology and metabolic engineering require the ability to alter the genome of the strain in question. Limited research has been conducted in strain engineering tools specific for Pantoea strains. Preliminary experimental results are promising for the application of Lambda-Red recombineering, which is an easy and efficient method for generating genetic modifications. In conclusion, this study aims to provide a base-level understanding of Pantoea as a genus and pave the way for its application in various fields.
Original languageEnglish
Title of host publicationThe Danish Microbiological Society Annual Congress 2023 : Abstract book
Number of pages1
PublisherThe Danish Microbiological Society
Publication date2023
Pages60-60
Article number58
Publication statusPublished - 2023
EventThe Danish Microbiological Society Annual Congress 2023 - Copenhagen, Denmark
Duration: 13 Nov 202313 Nov 2023

Conference

ConferenceThe Danish Microbiological Society Annual Congress 2023
Country/TerritoryDenmark
CityCopenhagen
Period13/11/202313/11/2023

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