Does labelling frequency affect N rhizodeposition assessment using the cotton-wick method?

S. Mahieu, J. Fustec, Erik Steen Jensen, Y. Crozat

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The aim of the present study was to test and improve the reliability of the 15N cotton-wick method for measuring soil N derived from plant rhizodeposition, a critical value for assessing belowground nitrogen input in field-grown legumes. The effects of the concentration of the 15N labelling solution and the feeding frequency on assessment of nitrogen rhizodeposition were studied in two greenhouse experiments using the field pea (Pisum sativum L.). Neither the method nor the feeding frequency altered plant biomass and N partitioning, and the method appeared well adapted for assessing the belowground contribution of field-grown legumes to the soil N pool. However, nitrogen rhizodeposition assessment was strongly influenced by the feeding frequency and the concentration of labelling solution. At pod-filling and maturity, despite similar root 15N enrichment, the fraction of plants' belowground nitrogen allocated to rhizodeposition in both Frisson pea and the non-nodulating isoline P2 was 20 to more than 50% higher when plants were labelled continuously than when they were labelled using fortnightly pulses. Our results suggest that when 15N root enrichment was high, nitrogen rhizodeposition was overestimated only for plants that were 15N-fed by fortnightly pulses, and not in plants 15N-fed continuously. This phenomenon was especially observed for plants that rely on symbiotic N2 fixation for N acquisition, and it may be linked to the concentration of the labelling solution. In conclusion, the assessment of nitrogen rhizodeposition was more reliable when plants were labelled continuously with a dilute solution of 15N urea.
    Original languageEnglish
    JournalSoil Biology & Biochemistry
    Volume41
    Issue number10
    Pages (from-to)2236-2243
    ISSN0038-0717
    DOIs
    Publication statusPublished - 2009

    Keywords

    • Bio energy
    • Bioenergy and biomass

    Cite this

    Mahieu, S. ; Fustec, J. ; Jensen, Erik Steen ; Crozat, Y. / Does labelling frequency affect N rhizodeposition assessment using the cotton-wick method?. In: Soil Biology & Biochemistry. 2009 ; Vol. 41, No. 10. pp. 2236-2243.
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    abstract = "The aim of the present study was to test and improve the reliability of the 15N cotton-wick method for measuring soil N derived from plant rhizodeposition, a critical value for assessing belowground nitrogen input in field-grown legumes. The effects of the concentration of the 15N labelling solution and the feeding frequency on assessment of nitrogen rhizodeposition were studied in two greenhouse experiments using the field pea (Pisum sativum L.). Neither the method nor the feeding frequency altered plant biomass and N partitioning, and the method appeared well adapted for assessing the belowground contribution of field-grown legumes to the soil N pool. However, nitrogen rhizodeposition assessment was strongly influenced by the feeding frequency and the concentration of labelling solution. At pod-filling and maturity, despite similar root 15N enrichment, the fraction of plants' belowground nitrogen allocated to rhizodeposition in both Frisson pea and the non-nodulating isoline P2 was 20 to more than 50{\%} higher when plants were labelled continuously than when they were labelled using fortnightly pulses. Our results suggest that when 15N root enrichment was high, nitrogen rhizodeposition was overestimated only for plants that were 15N-fed by fortnightly pulses, and not in plants 15N-fed continuously. This phenomenon was especially observed for plants that rely on symbiotic N2 fixation for N acquisition, and it may be linked to the concentration of the labelling solution. In conclusion, the assessment of nitrogen rhizodeposition was more reliable when plants were labelled continuously with a dilute solution of 15N urea.",
    keywords = "Bio energy, Bioenergy and biomass, Bioenergi, Biomasse og bioenergi",
    author = "S. Mahieu and J. Fustec and Jensen, {Erik Steen} and Y. Crozat",
    year = "2009",
    doi = "10.1016/j.soilbio.2009.08.008",
    language = "English",
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    Does labelling frequency affect N rhizodeposition assessment using the cotton-wick method? / Mahieu, S.; Fustec, J.; Jensen, Erik Steen; Crozat, Y.

    In: Soil Biology & Biochemistry, Vol. 41, No. 10, 2009, p. 2236-2243.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Does labelling frequency affect N rhizodeposition assessment using the cotton-wick method?

    AU - Mahieu, S.

    AU - Fustec, J.

    AU - Jensen, Erik Steen

    AU - Crozat, Y.

    PY - 2009

    Y1 - 2009

    N2 - The aim of the present study was to test and improve the reliability of the 15N cotton-wick method for measuring soil N derived from plant rhizodeposition, a critical value for assessing belowground nitrogen input in field-grown legumes. The effects of the concentration of the 15N labelling solution and the feeding frequency on assessment of nitrogen rhizodeposition were studied in two greenhouse experiments using the field pea (Pisum sativum L.). Neither the method nor the feeding frequency altered plant biomass and N partitioning, and the method appeared well adapted for assessing the belowground contribution of field-grown legumes to the soil N pool. However, nitrogen rhizodeposition assessment was strongly influenced by the feeding frequency and the concentration of labelling solution. At pod-filling and maturity, despite similar root 15N enrichment, the fraction of plants' belowground nitrogen allocated to rhizodeposition in both Frisson pea and the non-nodulating isoline P2 was 20 to more than 50% higher when plants were labelled continuously than when they were labelled using fortnightly pulses. Our results suggest that when 15N root enrichment was high, nitrogen rhizodeposition was overestimated only for plants that were 15N-fed by fortnightly pulses, and not in plants 15N-fed continuously. This phenomenon was especially observed for plants that rely on symbiotic N2 fixation for N acquisition, and it may be linked to the concentration of the labelling solution. In conclusion, the assessment of nitrogen rhizodeposition was more reliable when plants were labelled continuously with a dilute solution of 15N urea.

    AB - The aim of the present study was to test and improve the reliability of the 15N cotton-wick method for measuring soil N derived from plant rhizodeposition, a critical value for assessing belowground nitrogen input in field-grown legumes. The effects of the concentration of the 15N labelling solution and the feeding frequency on assessment of nitrogen rhizodeposition were studied in two greenhouse experiments using the field pea (Pisum sativum L.). Neither the method nor the feeding frequency altered plant biomass and N partitioning, and the method appeared well adapted for assessing the belowground contribution of field-grown legumes to the soil N pool. However, nitrogen rhizodeposition assessment was strongly influenced by the feeding frequency and the concentration of labelling solution. At pod-filling and maturity, despite similar root 15N enrichment, the fraction of plants' belowground nitrogen allocated to rhizodeposition in both Frisson pea and the non-nodulating isoline P2 was 20 to more than 50% higher when plants were labelled continuously than when they were labelled using fortnightly pulses. Our results suggest that when 15N root enrichment was high, nitrogen rhizodeposition was overestimated only for plants that were 15N-fed by fortnightly pulses, and not in plants 15N-fed continuously. This phenomenon was especially observed for plants that rely on symbiotic N2 fixation for N acquisition, and it may be linked to the concentration of the labelling solution. In conclusion, the assessment of nitrogen rhizodeposition was more reliable when plants were labelled continuously with a dilute solution of 15N urea.

    KW - Bio energy

    KW - Bioenergy and biomass

    KW - Bioenergi

    KW - Biomasse og bioenergi

    U2 - 10.1016/j.soilbio.2009.08.008

    DO - 10.1016/j.soilbio.2009.08.008

    M3 - Journal article

    VL - 41

    SP - 2236

    EP - 2243

    JO - Soil Biology & Biochemistry

    JF - Soil Biology & Biochemistry

    SN - 0038-0717

    IS - 10

    ER -