DNP Methods for Cardiac Metabolic Imaging with Hyperpolarized [1-13C]pyruvate Large Dose Injection in Pigs

Alessandra Flori, Francesca Frijia, Vincenzo Lionetti, Jan Henrik Ardenkjær-Larsen, Vincenzo Positano, Giulio Giovannetti, Rolf F. Schulte, Florian Wiesinger, Fabio A. Recchia, Luigi Landini, Maria F. Santarelli, Massimo Lombardi, Luca Menichetti

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Abstract

In this study we set up and optimized a dynamic nuclear polarization (DNP) procedure for the hyperpolarization of a large dose suitable for cardiac metabolic
imaging in vivo in swine models. The dose range proposed in this study allowed chemical shift imaging of cardiac metabolism with hyperpolarized [1-13C]pyruvate in pigs. We investigated the typical pattern of distribution of [1-13C]pyruvate and its downstream metabolites: we analysed the variation of the maximum value of the normalized [1-13C]pyruvate signal and the global pyruvate signal in left ventricle (LV). The study reports data obtained with a large dose increase compared to small animal studies: 20 ml of 230 mM [1-13C]pyruvate with 16 ± 3 % polarization (mean ± SD), using a DNP system operating at *1.4 K with a magnetic field of 3.35 T. A significant correlation between the maximum value of the normalized [1-13C]pyruvate signal and the global pyruvate signal in the LV was found. The characterization of the dynamic range of the signal and the optimization of the standardized dose could be a starting point for designing pathophysiological studies in experimental large animal models. With this approach the hyperpolarization of metabolic substrates could be applied in biomedical magnetic resonance, which could become one of the most promising models for cardiovascular imaging.
Original languageEnglish
JournalApplied Magnetic Resonance
Volume43
Issue number1-2
Pages (from-to)299-310
ISSN0937-9347
DOIs
Publication statusPublished - 2012
Externally publishedYes

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