DNA vaccination of rainbow trout against viral hemorrhagic septicemia virus: A dose-response and time-course study

Ellen Lorenzen, Katja Einer-Jensen, T. Martinussen, S.E. LaPatra, Niels Lorenzen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Viral hemorrhagic septicemia (VHS) in rainbow trout Oncorhynchus mykiss is caused by VHS virus (VHSV), which belongs to the rhabdovirus family. Among the different strategies for immunizing fish with a recombinant vaccine, genetic immunization has recently proven to be highly effective. To further investigate the potential for protecting fish against VHS by DNA vaccination, experiments were conducted to determine the amount of plasmid DNA needed for induction of protective immunity. The time to onset of immunity and the duration of protection following administration of a protective vaccine dose were also analyzed. The dose-response analysis revealed that significant protection of rainbow trout fingerlings was obtained following intramuscular injection of only 0.01 mug of plasmid DNA encoding the VHSV glycoprotein gene. In addition, higher doses of DNA induced immunity to a virus isolate serologically different from the isolate used for vaccine development. Following administration of 1 mug of a DNA vaccine, significant protection against VHS was observed in the fish as early as 8 d postvaccination. At 168 d postvaccination, the fish had increased in size by a factor of 10 and protection against a lethal dose of VHSV was still evident. The results confirm the great potential for DNA vaccination in inducing efficient immunoprophylaxis against viral diseases in aquacultured fish.
    Original languageEnglish
    JournalJournal of Aquatic Animal Health
    Volume12
    Issue number3
    Pages (from-to)167-180
    ISSN0899-7659
    Publication statusPublished - 2000

    Fingerprint Dive into the research topics of 'DNA vaccination of rainbow trout against viral hemorrhagic septicemia virus: A dose-response and time-course study'. Together they form a unique fingerprint.

    Cite this