DNA microarrays immobilized on unmodified plastics in a microfluidic biochip for rapid typing of Avian Influenza Virus

Sun Yi; Ivan R. Perch-Nielsen; Martin Dufva; David Sabourin; Dang Duong Bang; Jonas Høgberg; Minqiang Bu; Anders Wolff

doi:10.1109/TRANSDUCERS.2011.5969311

DNA microarrays immobilized on unmodified plastics in a microfluidic biochip for rapid typing of Avian Influenza Virus

Sun Yi, Ivan R. Perch-Nielsen, Martin Dufva, David Sabourin, Dang Duong Bang, Jonas Høgberg, Minqiang Bu, Anders Wolff

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Abstract

Polymers are widely used for microfluidic systems, but fabrication of microarrays on such materials often requires complicated chemical surface modifications, which hinders the integration of microarrays into microfluidic systems. In this paper, we demonstrate that UV irradiation can be used to directly immobilize poly(T)poly(C)-tagged DNA oligonucleotide probes on non-modified plastic surfaces. This one-step, cost-effective process provides very high immobilization and hybridization efficiencies and is applicable to many different types of polymers. Using this microarray fabrication technique, a portable cyclic olefin copolymer (COC) microarray device containing eight individually addressable microfluidic channels was developed for fast identification of Avian Influenza Virus (AIV) by DNA hybridization. This plastic biochip offers benefits of low fabrication cost and parallel processing of multiple samples, and could be used as a point-of-care device for clinical diagnostics and gene expression analysis.

Original language	English
Title of host publication	Transducers’11, Beijing, China, June 5-9, 2011
Publisher	IEEE
Publication date	2011
Pages	878-881
ISBN (Print)	978-1-4577-0157-3
DOIs	https://doi.org/10.1109/TRANSDUCERS.2011.5969311
Publication status	Published - 2011
Event	16th International Solid-State Sensors, Actuators and Microsystems Conference - Beijing, China Duration: 5 Jun 2011 → 9 Jun 2011 Conference number: 16 http://www.transducers11-beijing.org/

Conference

Conference	16th International Solid-State Sensors, Actuators and Microsystems Conference
Number	16
Country/Territory	China
City	Beijing
Period	05/06/2011 → 09/06/2011
Internet address	http://www.transducers11-beijing.org/

Keywords

Microfluidic
Microarray
Non-modified plastic
DNA immobilization
UV

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title = "DNA microarrays immobilized on unmodified plastics in a microfluidic biochip for rapid typing of Avian Influenza Virus",

abstract = "Polymers are widely used for microfluidic systems, but fabrication of microarrays on such materials often requires complicated chemical surface modifications, which hinders the integration of microarrays into microfluidic systems. In this paper, we demonstrate that UV irradiation can be used to directly immobilize poly(T)poly(C)-tagged DNA oligonucleotide probes on non-modified plastic surfaces. This one-step, cost-effective process provides very high immobilization and hybridization efficiencies and is applicable to many different types of polymers. Using this microarray fabrication technique, a portable cyclic olefin copolymer (COC) microarray device containing eight individually addressable microfluidic channels was developed for fast identification of Avian Influenza Virus (AIV) by DNA hybridization. This plastic biochip offers benefits of low fabrication cost and parallel processing of multiple samples, and could be used as a point-of-care device for clinical diagnostics and gene expression analysis.",

keywords = "Microfluidic, Microarray, Non-modified plastic, DNA immobilization, UV",

author = "Sun Yi and Perch-Nielsen, {Ivan R.} and Martin Dufva and David Sabourin and Bang, {Dang Duong} and Jonas H{\o}gberg and Minqiang Bu and Anders Wolff",

year = "2011",

doi = "10.1109/TRANSDUCERS.2011.5969311",

language = "English",

isbn = "978-1-4577-0157-3",

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booktitle = "Transducers{\textquoteright}11, Beijing, China, June 5-9, 2011",

publisher = "IEEE",

address = "United States",

note = "16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11 ; Conference date: 05-06-2011 Through 09-06-2011",

url = "http://www.transducers11-beijing.org/",

}

Yi, S, Perch-Nielsen, IR, Dufva, M, Sabourin, D, Bang, DD, Høgberg, J, Bu, M & Wolff, A 2011, DNA microarrays immobilized on unmodified plastics in a microfluidic biochip for rapid typing of Avian Influenza Virus. in Transducers’11, Beijing, China, June 5-9, 2011. IEEE, pp. 878-881, 16th International Solid-State Sensors, Actuators and Microsystems Conference, Beijing, China, 05/06/2011. https://doi.org/10.1109/TRANSDUCERS.2011.5969311

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T1 - DNA microarrays immobilized on unmodified plastics in a microfluidic biochip for rapid typing of Avian Influenza Virus

AU - Yi, Sun

AU - Perch-Nielsen, Ivan R.

AU - Dufva, Martin

AU - Sabourin, David

AU - Bang, Dang Duong

AU - Høgberg, Jonas

AU - Bu, Minqiang

AU - Wolff, Anders

N1 - Conference code: 16

PY - 2011

Y1 - 2011

N2 - Polymers are widely used for microfluidic systems, but fabrication of microarrays on such materials often requires complicated chemical surface modifications, which hinders the integration of microarrays into microfluidic systems. In this paper, we demonstrate that UV irradiation can be used to directly immobilize poly(T)poly(C)-tagged DNA oligonucleotide probes on non-modified plastic surfaces. This one-step, cost-effective process provides very high immobilization and hybridization efficiencies and is applicable to many different types of polymers. Using this microarray fabrication technique, a portable cyclic olefin copolymer (COC) microarray device containing eight individually addressable microfluidic channels was developed for fast identification of Avian Influenza Virus (AIV) by DNA hybridization. This plastic biochip offers benefits of low fabrication cost and parallel processing of multiple samples, and could be used as a point-of-care device for clinical diagnostics and gene expression analysis.

AB - Polymers are widely used for microfluidic systems, but fabrication of microarrays on such materials often requires complicated chemical surface modifications, which hinders the integration of microarrays into microfluidic systems. In this paper, we demonstrate that UV irradiation can be used to directly immobilize poly(T)poly(C)-tagged DNA oligonucleotide probes on non-modified plastic surfaces. This one-step, cost-effective process provides very high immobilization and hybridization efficiencies and is applicable to many different types of polymers. Using this microarray fabrication technique, a portable cyclic olefin copolymer (COC) microarray device containing eight individually addressable microfluidic channels was developed for fast identification of Avian Influenza Virus (AIV) by DNA hybridization. This plastic biochip offers benefits of low fabrication cost and parallel processing of multiple samples, and could be used as a point-of-care device for clinical diagnostics and gene expression analysis.

KW - Microfluidic

KW - Microarray

KW - Non-modified plastic

KW - DNA immobilization

KW - UV

U2 - 10.1109/TRANSDUCERS.2011.5969311

DO - 10.1109/TRANSDUCERS.2011.5969311

M3 - Article in proceedings

SN - 978-1-4577-0157-3

SP - 878

EP - 881

BT - Transducers’11, Beijing, China, June 5-9, 2011

PB - IEEE

T2 - 16th International Solid-State Sensors, Actuators and Microsystems Conference

Y2 - 5 June 2011 through 9 June 2011

ER -

DNA microarrays immobilized on unmodified plastics in a microfluidic biochip for rapid typing of Avian Influenza Virus

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