DNA microarrays immobilized on unmodified plastics in a microfluidic biochip for rapid typing of Avian Influenza Virus

Sun Yi, Ivan R. Perch-Nielsen, Martin Dufva, David Sabourin, Dang Duong Bang, Jonas Høgberg, Minqiang Bu, Anders Wolff

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    Abstract

    Polymers are widely used for microfluidic systems, but fabrication of microarrays on such materials often requires complicated chemical surface modifications, which hinders the integration of microarrays into microfluidic systems. In this paper, we demonstrate that UV irradiation can be used to directly immobilize poly(T)poly(C)-tagged DNA oligonucleotide probes on non-modified plastic surfaces. This one-step, cost-effective process provides very high immobilization and hybridization efficiencies and is applicable to many different types of polymers. Using this microarray fabrication technique, a portable cyclic olefin copolymer (COC) microarray device containing eight individually addressable microfluidic channels was developed for fast identification of Avian Influenza Virus (AIV) by DNA hybridization. This plastic biochip offers benefits of low fabrication cost and parallel processing of multiple samples, and could be used as a point-of-care device for clinical diagnostics and gene expression analysis.
    Original languageEnglish
    Title of host publicationTransducers’11, Beijing, China, June 5-9, 2011
    PublisherIEEE
    Publication date2011
    Pages878-881
    ISBN (Print)978-1-4577-0157-3
    DOIs
    Publication statusPublished - 2011
    Event16th International Solid-State Sensors, Actuators and Microsystems Conference - Beijing, China
    Duration: 5 Jun 20119 Jun 2011
    Conference number: 16
    http://www.transducers11-beijing.org/

    Conference

    Conference16th International Solid-State Sensors, Actuators and Microsystems Conference
    Number16
    CountryChina
    CityBeijing
    Period05/06/201109/06/2011
    Internet address

    Keywords

    • Microfluidic
    • Microarray
    • Non-modified plastic
    • DNA immobilization
    • UV

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