DNA microarray analysis of fim mutations in Escherichia coli

Mark Schembri, David Ussery, Christopher Workman, Henrik Hasman, Per Klemm

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Bacterial adhesion is often mediated by complex polymeric surface structures referred to as fimbriae. Type I fimbriae of Escherichia coli represent the archetypical and best characterised fimbrial system. These adhesive organelles mediate binding to D-mannose and are directly associated with virulence in the urinary tract. A typical type I fimbriated bacterium has up to 500 fimbriae on its surface, with each fimbria consisting of similar to1000 individual subunits. This equates to approximately 8% of the total cellular protein and is potentially a significant resource drain for the cell. Here we have used DNA microarray analysis to examine the molecular events involved in response to fimbrial gene expression in E. coli K-12. Observed differential expression levels of the fim genes were in good agreement with our current knowledge of the stoichiometry of type I fimbriae. Changes in fim expression correlated directly with alterations in colony morphology. Deletion of the entire fim gene cluster resulted in the converse expression of another surface protein Antigen 43 (Ag43). Specific deletion of the film gene did not affect expression of other film genes or Ag43, but did dramatically reduce the number of fimbriae expressed on the cell surface. The use of high-resolution oligonucleotide arrays for defining points of transcription initiation and termination is also demonstrated.
    Original languageEnglish
    JournalMolecular Genetics and Genomics
    Volume267
    Issue number6
    Pages (from-to)721-729
    ISSN1617-4615
    Publication statusPublished - 2002

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