DNA hybridization sensing for cytogenetic analysis

Dorota Kwasny, Johannes Dapra, Anna Line Brøgger, Filippo Bosco, Zeynep Tumer, Maria Dimaki, Noemi Rozlosnik, Anja Boisen, Winnie Edith Svendsen

    Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

    Abstract

    Cytogenetic analysis focuses on studying the cell structure, mainly in respect to chromosome content and their structure. Chromosome abnormalities, such as translocations may cause various genetic disorders, but are also associated with heametological malignancies. Chromosome translocations are rearrangements between two chromosome arms that results in two derivative chromosomes having a mixed DNA sequence. The current detection method is a Fluorescent In situ Hybridization, which requires a use of expensive, fluorescently labeled probes that target the DNA sequences of two chromosomes involved in the translocation (Kwasny et al., 2012).
    We have developed a new double hybridization assay that allows for sorting of the DNA chromosomal fragments into separate compartment, moreover allowing for detection of the translocation. To detect the translocation it is necessary to determine that the two DNA sequences forming a derivative chromosome are connected, which is achieved by two subsequent hybridization steps. The first example of the translocation detection was presented on lab-on-a-disc using fluorescently labeled DNA fragments, representing the derivative chromosome (Brøgger et al., 2012). To allow for cheaper detection a label-free approach has been investigated using electrochemical impedance spectroscopy as a sensing method. We present here our recent results in regards to DNA sensing on metallic and conductive polymer electrodes for translocation detection. Our sensors are inexpensive and can be successfully applied in cytogenetic analysis as a replacement of standard techniques.
    Original languageEnglish
    Title of host publicationProceedings of Analytix 2013
    Publication date2013
    Publication statusPublished - 2013
    EventAnalytix 2013 - Suzhou, China
    Duration: 21 Mar 201323 Mar 2013
    Conference number: 2

    Conference

    ConferenceAnalytix 2013
    Number2
    CountryChina
    CitySuzhou
    Period21/03/201323/03/2013

    Cite this

    Kwasny, D., Dapra, J., Brøgger, A. L., Bosco, F., Tumer, Z., Dimaki, M., ... Svendsen, W. E. (2013). DNA hybridization sensing for cytogenetic analysis. In Proceedings of Analytix 2013
    Kwasny, Dorota ; Dapra, Johannes ; Brøgger, Anna Line ; Bosco, Filippo ; Tumer, Zeynep ; Dimaki, Maria ; Rozlosnik, Noemi ; Boisen, Anja ; Svendsen, Winnie Edith. / DNA hybridization sensing for cytogenetic analysis. Proceedings of Analytix 2013. 2013.
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    title = "DNA hybridization sensing for cytogenetic analysis",
    abstract = "Cytogenetic analysis focuses on studying the cell structure, mainly in respect to chromosome content and their structure. Chromosome abnormalities, such as translocations may cause various genetic disorders, but are also associated with heametological malignancies. Chromosome translocations are rearrangements between two chromosome arms that results in two derivative chromosomes having a mixed DNA sequence. The current detection method is a Fluorescent In situ Hybridization, which requires a use of expensive, fluorescently labeled probes that target the DNA sequences of two chromosomes involved in the translocation (Kwasny et al., 2012).We have developed a new double hybridization assay that allows for sorting of the DNA chromosomal fragments into separate compartment, moreover allowing for detection of the translocation. To detect the translocation it is necessary to determine that the two DNA sequences forming a derivative chromosome are connected, which is achieved by two subsequent hybridization steps. The first example of the translocation detection was presented on lab-on-a-disc using fluorescently labeled DNA fragments, representing the derivative chromosome (Br{\o}gger et al., 2012). To allow for cheaper detection a label-free approach has been investigated using electrochemical impedance spectroscopy as a sensing method. We present here our recent results in regards to DNA sensing on metallic and conductive polymer electrodes for translocation detection. Our sensors are inexpensive and can be successfully applied in cytogenetic analysis as a replacement of standard techniques.",
    author = "Dorota Kwasny and Johannes Dapra and Br{\o}gger, {Anna Line} and Filippo Bosco and Zeynep Tumer and Maria Dimaki and Noemi Rozlosnik and Anja Boisen and Svendsen, {Winnie Edith}",
    year = "2013",
    language = "English",
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    Kwasny, D, Dapra, J, Brøgger, AL, Bosco, F, Tumer, Z, Dimaki, M, Rozlosnik, N, Boisen, A & Svendsen, WE 2013, DNA hybridization sensing for cytogenetic analysis. in Proceedings of Analytix 2013. Analytix 2013, Suzhou, China, 21/03/2013.

    DNA hybridization sensing for cytogenetic analysis. / Kwasny, Dorota; Dapra, Johannes; Brøgger, Anna Line; Bosco, Filippo; Tumer, Zeynep; Dimaki, Maria; Rozlosnik, Noemi; Boisen, Anja; Svendsen, Winnie Edith.

    Proceedings of Analytix 2013. 2013.

    Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

    TY - ABST

    T1 - DNA hybridization sensing for cytogenetic analysis

    AU - Kwasny, Dorota

    AU - Dapra, Johannes

    AU - Brøgger, Anna Line

    AU - Bosco, Filippo

    AU - Tumer, Zeynep

    AU - Dimaki, Maria

    AU - Rozlosnik, Noemi

    AU - Boisen, Anja

    AU - Svendsen, Winnie Edith

    PY - 2013

    Y1 - 2013

    N2 - Cytogenetic analysis focuses on studying the cell structure, mainly in respect to chromosome content and their structure. Chromosome abnormalities, such as translocations may cause various genetic disorders, but are also associated with heametological malignancies. Chromosome translocations are rearrangements between two chromosome arms that results in two derivative chromosomes having a mixed DNA sequence. The current detection method is a Fluorescent In situ Hybridization, which requires a use of expensive, fluorescently labeled probes that target the DNA sequences of two chromosomes involved in the translocation (Kwasny et al., 2012).We have developed a new double hybridization assay that allows for sorting of the DNA chromosomal fragments into separate compartment, moreover allowing for detection of the translocation. To detect the translocation it is necessary to determine that the two DNA sequences forming a derivative chromosome are connected, which is achieved by two subsequent hybridization steps. The first example of the translocation detection was presented on lab-on-a-disc using fluorescently labeled DNA fragments, representing the derivative chromosome (Brøgger et al., 2012). To allow for cheaper detection a label-free approach has been investigated using electrochemical impedance spectroscopy as a sensing method. We present here our recent results in regards to DNA sensing on metallic and conductive polymer electrodes for translocation detection. Our sensors are inexpensive and can be successfully applied in cytogenetic analysis as a replacement of standard techniques.

    AB - Cytogenetic analysis focuses on studying the cell structure, mainly in respect to chromosome content and their structure. Chromosome abnormalities, such as translocations may cause various genetic disorders, but are also associated with heametological malignancies. Chromosome translocations are rearrangements between two chromosome arms that results in two derivative chromosomes having a mixed DNA sequence. The current detection method is a Fluorescent In situ Hybridization, which requires a use of expensive, fluorescently labeled probes that target the DNA sequences of two chromosomes involved in the translocation (Kwasny et al., 2012).We have developed a new double hybridization assay that allows for sorting of the DNA chromosomal fragments into separate compartment, moreover allowing for detection of the translocation. To detect the translocation it is necessary to determine that the two DNA sequences forming a derivative chromosome are connected, which is achieved by two subsequent hybridization steps. The first example of the translocation detection was presented on lab-on-a-disc using fluorescently labeled DNA fragments, representing the derivative chromosome (Brøgger et al., 2012). To allow for cheaper detection a label-free approach has been investigated using electrochemical impedance spectroscopy as a sensing method. We present here our recent results in regards to DNA sensing on metallic and conductive polymer electrodes for translocation detection. Our sensors are inexpensive and can be successfully applied in cytogenetic analysis as a replacement of standard techniques.

    M3 - Conference abstract in proceedings

    BT - Proceedings of Analytix 2013

    ER -

    Kwasny D, Dapra J, Brøgger AL, Bosco F, Tumer Z, Dimaki M et al. DNA hybridization sensing for cytogenetic analysis. In Proceedings of Analytix 2013. 2013