We have developed a new double hybridization assay that allows for sorting of the DNA chromosomal fragments into separate compartment, moreover allowing for detection of the translocation. To detect the translocation it is necessary to determine that the two DNA sequences forming a derivative chromosome are connected, which is achieved by two subsequent hybridization steps. The first example of the translocation detection was presented on lab-on-a-disc using fluorescently labeled DNA fragments, representing the derivative chromosome (Brøgger et al., 2012). To allow for cheaper detection a label-free approach has been investigated using electrochemical impedance spectroscopy as a sensing method. We present here our recent results in regards to DNA sensing on metallic and conductive polymer electrodes for translocation detection. Our sensors are inexpensive and can be successfully applied in cytogenetic analysis as a replacement of standard techniques.
|Title of host publication||Proceedings of Analytix 2013|
|Publication status||Published - 2013|
|Event||Analytix 2013 - Suzhou, China|
Duration: 21 Mar 2013 → 23 Mar 2013
Conference number: 2
|Period||21/03/2013 → 23/03/2013|