DNA damage in mouse organs and in human sperm cells by bisphenol A

Anoop Kumar Sharma*, Julie Boberg, Marianne Dybdahl

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

The in vivo genotoxic potential of bisphenol A using the comet assay in mice and in human sperm cells in vitro without metabolizing enzymes was studied. Male mice were exposed by oral gavage to the following doses of bisphenol A (0,125, 250 and 500 mg/kg body weight). DNA damage was investigated in liver, kidney, testes, urinary bladder, colon and lungs cells. In testicular cells, a significant increase in DNA strand breaks was observed in the lowest, but not in the medium or highest dose groups. Histopathological investigation of the testicular samples did not show any treatment dose-related effects. No DNA strand breaks were observed in any of the other investigated tissues. In human sperm cells in vitro, bisphenol A did not induce DNA strand breaks.
Original languageEnglish
JournalToxicological and Environmental Chemistry
Volume100
Issue number4
Pages (from-to)465-478
Number of pages14
ISSN0277-2248
DOIs
Publication statusPublished - 2018

Keywords

  • DNA damage
  • Comet assay
  • Mice
  • Testes
  • Human sperm cells

Cite this

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title = "DNA damage in mouse organs and in human sperm cells by bisphenol A",
abstract = "The in vivo genotoxic potential of bisphenol A using the comet assay in mice and in human sperm cells in vitro without metabolizing enzymes was studied. Male mice were exposed by oral gavage to the following doses of bisphenol A (0,125, 250 and 500 mg/kg body weight). DNA damage was investigated in liver, kidney, testes, urinary bladder, colon and lungs cells. In testicular cells, a significant increase in DNA strand breaks was observed in the lowest, but not in the medium or highest dose groups. Histopathological investigation of the testicular samples did not show any treatment dose-related effects. No DNA strand breaks were observed in any of the other investigated tissues. In human sperm cells in vitro, bisphenol A did not induce DNA strand breaks.",
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author = "Sharma, {Anoop Kumar} and Julie Boberg and Marianne Dybdahl",
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DNA damage in mouse organs and in human sperm cells by bisphenol A. / Sharma, Anoop Kumar; Boberg, Julie; Dybdahl, Marianne.

In: Toxicological and Environmental Chemistry, Vol. 100, No. 4, 2018, p. 465-478.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - DNA damage in mouse organs and in human sperm cells by bisphenol A

AU - Sharma, Anoop Kumar

AU - Boberg, Julie

AU - Dybdahl, Marianne

PY - 2018

Y1 - 2018

N2 - The in vivo genotoxic potential of bisphenol A using the comet assay in mice and in human sperm cells in vitro without metabolizing enzymes was studied. Male mice were exposed by oral gavage to the following doses of bisphenol A (0,125, 250 and 500 mg/kg body weight). DNA damage was investigated in liver, kidney, testes, urinary bladder, colon and lungs cells. In testicular cells, a significant increase in DNA strand breaks was observed in the lowest, but not in the medium or highest dose groups. Histopathological investigation of the testicular samples did not show any treatment dose-related effects. No DNA strand breaks were observed in any of the other investigated tissues. In human sperm cells in vitro, bisphenol A did not induce DNA strand breaks.

AB - The in vivo genotoxic potential of bisphenol A using the comet assay in mice and in human sperm cells in vitro without metabolizing enzymes was studied. Male mice were exposed by oral gavage to the following doses of bisphenol A (0,125, 250 and 500 mg/kg body weight). DNA damage was investigated in liver, kidney, testes, urinary bladder, colon and lungs cells. In testicular cells, a significant increase in DNA strand breaks was observed in the lowest, but not in the medium or highest dose groups. Histopathological investigation of the testicular samples did not show any treatment dose-related effects. No DNA strand breaks were observed in any of the other investigated tissues. In human sperm cells in vitro, bisphenol A did not induce DNA strand breaks.

KW - DNA damage

KW - Comet assay

KW - Mice

KW - Testes

KW - Human sperm cells

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JO - Toxicological and Environmental Chemistry

JF - Toxicological and Environmental Chemistry

SN - 0277-2248

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