DNA-Based Identification and Chemical Characteristics of Hypnea musciformis from Coastal Sites in Ghana

Marcel Tutor Ale, Kristian Barrett, Gloria Addico, Nanna Rhein-Knudsen, Amoako deGraft-Johnson, Anne S. Meyer

Research output: Contribution to journalJournal articleResearchpeer-review

270 Downloads (Pure)

Abstract

This work reveals new, important insights about the influence of broad spatial variationson the phylogenetic relationship and chemical characteristics of Ghanaian Hypnea musciformis—acarrageenan-containing red seaweed. DNA barcoding techniques alleviate the difficulty for accurate morphological identification. COI barcode sequences of the Ghanaian H. musciformis showed <0.7% intraspecies divergence, indicating no distinct phylogenetic variation, suggesting that they actually belong to the same species. Thus, the spatial distribution of the sampling sites along the coast of Ghana did not influence the phylogenetic characteristics of H. musciformis in the region. The data also showed that the Ghanaian Hypnea sp. examined in this work should be regarded as the same species as the H. musciformis collected in Brazilian Sao Paulo (KP725276) with only 0.8%–1.3% intraspecies divergence. However, the comparison of COI sequences of Ghanaian H. musciformis with the available COI sequence of H. musciformis from other countries showed intraspecies divergences of0%–6.9% indicating that the COI sequences for H. musciformis in the GenBank may include different subspecies. Although samples did not differ phylogenetically, the chemical characteristics of the H. musciformis differed significantly between different sampling locations in Ghana. The levels ofthe monosaccharides, notably galactose (20%–30% dw) and glucose (10%–18% dw), as well as the seawater inorganic salt concentration (21–32 mg/L) and ash content (19%–33% dw), varied between H. musciformis collected at different coastal locations in Ghana. The current work demonstrated that DNA-based identification allowed a detailed understanding of H. musciformis phylogenetic characteristics and revealed that chemical compositional differences of H. musciformis occur along the Ghanaian coast which are not coupled with genetic variations among those samples.
Original languageEnglish
JournalDiversity
Volume8
Issue number2
Number of pages14
ISSN1424-2818
DOIs
Publication statusPublished - 2016

Bibliographical note

© 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

Keywords

  • DNA barcodes
  • LSU
  • UPA
  • COI
  • Hypnea musciformis
  • Marine biodiversity
  • Carrageenan
  • Spatial variation
  • Seaweed
  • Phylogenetic
  • Ghana

Cite this

@article{5b352433b32047dc8450eeb9b1256a46,
title = "DNA-Based Identification and Chemical Characteristics of Hypnea musciformis from Coastal Sites in Ghana",
abstract = "This work reveals new, important insights about the influence of broad spatial variationson the phylogenetic relationship and chemical characteristics of Ghanaian Hypnea musciformis—acarrageenan-containing red seaweed. DNA barcoding techniques alleviate the difficulty for accurate morphological identification. COI barcode sequences of the Ghanaian H. musciformis showed <0.7{\%} intraspecies divergence, indicating no distinct phylogenetic variation, suggesting that they actually belong to the same species. Thus, the spatial distribution of the sampling sites along the coast of Ghana did not influence the phylogenetic characteristics of H. musciformis in the region. The data also showed that the Ghanaian Hypnea sp. examined in this work should be regarded as the same species as the H. musciformis collected in Brazilian Sao Paulo (KP725276) with only 0.8{\%}–1.3{\%} intraspecies divergence. However, the comparison of COI sequences of Ghanaian H. musciformis with the available COI sequence of H. musciformis from other countries showed intraspecies divergences of0{\%}–6.9{\%} indicating that the COI sequences for H. musciformis in the GenBank may include different subspecies. Although samples did not differ phylogenetically, the chemical characteristics of the H. musciformis differed significantly between different sampling locations in Ghana. The levels ofthe monosaccharides, notably galactose (20{\%}–30{\%} dw) and glucose (10{\%}–18{\%} dw), as well as the seawater inorganic salt concentration (21–32 mg/L) and ash content (19{\%}–33{\%} dw), varied between H. musciformis collected at different coastal locations in Ghana. The current work demonstrated that DNA-based identification allowed a detailed understanding of H. musciformis phylogenetic characteristics and revealed that chemical compositional differences of H. musciformis occur along the Ghanaian coast which are not coupled with genetic variations among those samples.",
keywords = "DNA barcodes, LSU, UPA, COI, Hypnea musciformis, Marine biodiversity, Carrageenan, Spatial variation, Seaweed, Phylogenetic, Ghana",
author = "Ale, {Marcel Tutor} and Kristian Barrett and Gloria Addico and Nanna Rhein-Knudsen and Amoako deGraft-Johnson and Meyer, {Anne S.}",
note = "{\circledC} 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).",
year = "2016",
doi = "10.3390/d8020014",
language = "English",
volume = "8",
journal = "Diversity",
issn = "1424-2818",
publisher = "M D P I AG",
number = "2",

}

DNA-Based Identification and Chemical Characteristics of Hypnea musciformis from Coastal Sites in Ghana. / Ale, Marcel Tutor; Barrett, Kristian; Addico, Gloria; Rhein-Knudsen, Nanna; deGraft-Johnson, Amoako; Meyer, Anne S.

In: Diversity, Vol. 8, No. 2, 2016.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - DNA-Based Identification and Chemical Characteristics of Hypnea musciformis from Coastal Sites in Ghana

AU - Ale, Marcel Tutor

AU - Barrett, Kristian

AU - Addico, Gloria

AU - Rhein-Knudsen, Nanna

AU - deGraft-Johnson, Amoako

AU - Meyer, Anne S.

N1 - © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

PY - 2016

Y1 - 2016

N2 - This work reveals new, important insights about the influence of broad spatial variationson the phylogenetic relationship and chemical characteristics of Ghanaian Hypnea musciformis—acarrageenan-containing red seaweed. DNA barcoding techniques alleviate the difficulty for accurate morphological identification. COI barcode sequences of the Ghanaian H. musciformis showed <0.7% intraspecies divergence, indicating no distinct phylogenetic variation, suggesting that they actually belong to the same species. Thus, the spatial distribution of the sampling sites along the coast of Ghana did not influence the phylogenetic characteristics of H. musciformis in the region. The data also showed that the Ghanaian Hypnea sp. examined in this work should be regarded as the same species as the H. musciformis collected in Brazilian Sao Paulo (KP725276) with only 0.8%–1.3% intraspecies divergence. However, the comparison of COI sequences of Ghanaian H. musciformis with the available COI sequence of H. musciformis from other countries showed intraspecies divergences of0%–6.9% indicating that the COI sequences for H. musciformis in the GenBank may include different subspecies. Although samples did not differ phylogenetically, the chemical characteristics of the H. musciformis differed significantly between different sampling locations in Ghana. The levels ofthe monosaccharides, notably galactose (20%–30% dw) and glucose (10%–18% dw), as well as the seawater inorganic salt concentration (21–32 mg/L) and ash content (19%–33% dw), varied between H. musciformis collected at different coastal locations in Ghana. The current work demonstrated that DNA-based identification allowed a detailed understanding of H. musciformis phylogenetic characteristics and revealed that chemical compositional differences of H. musciformis occur along the Ghanaian coast which are not coupled with genetic variations among those samples.

AB - This work reveals new, important insights about the influence of broad spatial variationson the phylogenetic relationship and chemical characteristics of Ghanaian Hypnea musciformis—acarrageenan-containing red seaweed. DNA barcoding techniques alleviate the difficulty for accurate morphological identification. COI barcode sequences of the Ghanaian H. musciformis showed <0.7% intraspecies divergence, indicating no distinct phylogenetic variation, suggesting that they actually belong to the same species. Thus, the spatial distribution of the sampling sites along the coast of Ghana did not influence the phylogenetic characteristics of H. musciformis in the region. The data also showed that the Ghanaian Hypnea sp. examined in this work should be regarded as the same species as the H. musciformis collected in Brazilian Sao Paulo (KP725276) with only 0.8%–1.3% intraspecies divergence. However, the comparison of COI sequences of Ghanaian H. musciformis with the available COI sequence of H. musciformis from other countries showed intraspecies divergences of0%–6.9% indicating that the COI sequences for H. musciformis in the GenBank may include different subspecies. Although samples did not differ phylogenetically, the chemical characteristics of the H. musciformis differed significantly between different sampling locations in Ghana. The levels ofthe monosaccharides, notably galactose (20%–30% dw) and glucose (10%–18% dw), as well as the seawater inorganic salt concentration (21–32 mg/L) and ash content (19%–33% dw), varied between H. musciformis collected at different coastal locations in Ghana. The current work demonstrated that DNA-based identification allowed a detailed understanding of H. musciformis phylogenetic characteristics and revealed that chemical compositional differences of H. musciformis occur along the Ghanaian coast which are not coupled with genetic variations among those samples.

KW - DNA barcodes

KW - LSU

KW - UPA

KW - COI

KW - Hypnea musciformis

KW - Marine biodiversity

KW - Carrageenan

KW - Spatial variation

KW - Seaweed

KW - Phylogenetic

KW - Ghana

U2 - 10.3390/d8020014

DO - 10.3390/d8020014

M3 - Journal article

VL - 8

JO - Diversity

JF - Diversity

SN - 1424-2818

IS - 2

ER -