DNA barcoding via counterstaining with AT/GC sensitive ligands in injection-molded all-polymer nanochannel devices

Peter Friis Østergaard, Marco Matteucci, Walter Reisner, Rafael Taboryski

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Nanochannel technology, coupled with a suitable DNA labeling chemistry, is a powerful approach for performing high-throughput single-molecule mapping of genomes. Yet so far nanochannel technology has remained inaccessible to the broader research community due to high fabrication cost and/or requirement of specialized facilities/skill-sets. In this article we show that nanochannel-based mapping can be performed in all polymer chips fabricated via injection molding: a fabrication process so inexpensive that the devices can be considered disposable. Fluorescent intensity variations can be obtained from molecules extended in the polymer nanochannels via chemical counterstaining against YOYO-1. In particular, we demonstrate that the counterstaining induced fluorescent intensity variations to a large degree appear to be proportional to the theoretically computed sequence-maps of both local AT and GC variation along DNA sequences.
    Original languageEnglish
    JournalAnalyst
    Volume138
    Issue number4
    Pages (from-to)1249-1255
    ISSN0003-2654
    DOIs
    Publication statusPublished - 2013

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