DNA and origin region segregation are not affected by the transition from rod to sphere after inhibition of Escherichia coli MreB by A22

Aneta Karczmarek, Rocio Martinez-Arteaga, Svetlana Alexeeva, Flemming G. Hansen, Miguel Vicente, Nanne Nanninga, Tanneke den Blaauwen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The bacterial actin homologue MreB forms a helix underneath the cytoplasmic membrane and was shown to be essential in the morphogenesis of the rod-shaped cells. Additionally, MreB was implicated to be involved in DNA segregation. However, in our hands the mreBCD deletion strain (PA340-678) grew without apparent DNA segregation defect, suggesting that the reported chromosome segregation inhibition could be caused by a temporarily effect of MreB inhibition or depletion. To assess the involvement of MreB in DNA segregation during the transition from rod to sphere, we compared the effect of A22 and the PBP2 inhibitor mecillinam on the percentage of cells with segregated nucleoids and the number of oriC foci in wild-type Escherichia coli cells. Cells became spherical in the same time window during both treatments and we could not detect any difference in the chromosome or oriC segregation between these two treatments. Additionally, flow cytometric analyses showed that A22 and mecillinam treatment gave essentially the same chromosome segregation pattern. We conclude that MreB is not directly involved in DNA segregation of E. coli.
    Original languageEnglish
    JournalMolecular Microbiology
    Volume65
    Issue number1
    Pages (from-to)51-63
    ISSN0950-382X
    DOIs
    Publication statusPublished - 2007

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