Diversity of Flavobacterium psychrophilum and the potential use of its phages for protection against bacterial cold water disease in salmonids

D. Castillo, G. Higuera, M. Villa, M. Middelboe, Inger Dalsgaard, Lone Madsen, R. T. Espejo

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Flavobacterium psychrophilum causes rainbow trout fry syndrome (RTFS) and cold water disease (CWD) in salmonid aquaculture. We report characterization of F. psychrophilum strains and their bacteriophages isolated in Chilean salmonid aquaculture. Results suggest that under laboratory conditions phages can decrease mortality of salmonids from infection by their F. psychrophilum host strain. Twelve F. psychrophilum isolates were characterized, with DNA restriction patterns showing low diversity between strains despite their being obtained from different salmonid production sites and from different tissues. We isolated 15 bacteriophages able to infect some of the F. psychrophilum isolates and characterized six of them in detail. DNA genome sizes were close to 50 Kbp and corresponded to the Siphoviridae and Podoviridae families. One isolate, 6H, probably contains lipids as an essential virion component, based on its chloroform sensitivity and low buoyant density in CsCl. Each phage isolate rarely infected F. psychrophilum strains other than the strain used for its enrichment and isolation. Some bacteriophages could decrease mortality from intraperitoneal injection of its host strain when added together with the bacteria in a ratio of 10 plaque‐forming units per colony‐forming unit. While we recognize the artificial laboratory conditions used for these protection assays, this work is the first to demonstrate that phages might be able protect salmonids from RTFS or CWD.
    Original languageEnglish
    JournalJournal of Fish Diseases
    Volume35
    Issue number3
    Pages (from-to)193-201
    ISSN0140-7775
    DOIs
    Publication statusPublished - 2012

    Keywords

    • Phages salmonids
    • Flavobacterium psychrophilum
    • Bacterial cold water disease
    • Protection

    Cite this

    @article{746ac0c6a2ce4038887e7e4df3e338b2,
    title = "Diversity of Flavobacterium psychrophilum and the potential use of its phages for protection against bacterial cold water disease in salmonids",
    abstract = "Flavobacterium psychrophilum causes rainbow trout fry syndrome (RTFS) and cold water disease (CWD) in salmonid aquaculture. We report characterization of F. psychrophilum strains and their bacteriophages isolated in Chilean salmonid aquaculture. Results suggest that under laboratory conditions phages can decrease mortality of salmonids from infection by their F. psychrophilum host strain. Twelve F. psychrophilum isolates were characterized, with DNA restriction patterns showing low diversity between strains despite their being obtained from different salmonid production sites and from different tissues. We isolated 15 bacteriophages able to infect some of the F. psychrophilum isolates and characterized six of them in detail. DNA genome sizes were close to 50 Kbp and corresponded to the Siphoviridae and Podoviridae families. One isolate, 6H, probably contains lipids as an essential virion component, based on its chloroform sensitivity and low buoyant density in CsCl. Each phage isolate rarely infected F. psychrophilum strains other than the strain used for its enrichment and isolation. Some bacteriophages could decrease mortality from intraperitoneal injection of its host strain when added together with the bacteria in a ratio of 10 plaque‐forming units per colony‐forming unit. While we recognize the artificial laboratory conditions used for these protection assays, this work is the first to demonstrate that phages might be able protect salmonids from RTFS or CWD.",
    keywords = "Phages salmonids, Flavobacterium psychrophilum, Bacterial cold water disease, Protection",
    author = "D. Castillo and G. Higuera and M. Villa and M. Middelboe and Inger Dalsgaard and Lone Madsen and Espejo, {R. T.}",
    year = "2012",
    doi = "10.1111/j.1365-2761.2011.01336.x",
    language = "English",
    volume = "35",
    pages = "193--201",
    journal = "Journal of Fish Diseases",
    issn = "0140-7775",
    publisher = "Wiley-Blackwell",
    number = "3",

    }

    Diversity of Flavobacterium psychrophilum and the potential use of its phages for protection against bacterial cold water disease in salmonids. / Castillo, D.; Higuera, G.; Villa, M.; Middelboe, M.; Dalsgaard, Inger; Madsen, Lone; Espejo, R. T.

    In: Journal of Fish Diseases, Vol. 35, No. 3, 2012, p. 193-201.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Diversity of Flavobacterium psychrophilum and the potential use of its phages for protection against bacterial cold water disease in salmonids

    AU - Castillo, D.

    AU - Higuera, G.

    AU - Villa, M.

    AU - Middelboe, M.

    AU - Dalsgaard, Inger

    AU - Madsen, Lone

    AU - Espejo, R. T.

    PY - 2012

    Y1 - 2012

    N2 - Flavobacterium psychrophilum causes rainbow trout fry syndrome (RTFS) and cold water disease (CWD) in salmonid aquaculture. We report characterization of F. psychrophilum strains and their bacteriophages isolated in Chilean salmonid aquaculture. Results suggest that under laboratory conditions phages can decrease mortality of salmonids from infection by their F. psychrophilum host strain. Twelve F. psychrophilum isolates were characterized, with DNA restriction patterns showing low diversity between strains despite their being obtained from different salmonid production sites and from different tissues. We isolated 15 bacteriophages able to infect some of the F. psychrophilum isolates and characterized six of them in detail. DNA genome sizes were close to 50 Kbp and corresponded to the Siphoviridae and Podoviridae families. One isolate, 6H, probably contains lipids as an essential virion component, based on its chloroform sensitivity and low buoyant density in CsCl. Each phage isolate rarely infected F. psychrophilum strains other than the strain used for its enrichment and isolation. Some bacteriophages could decrease mortality from intraperitoneal injection of its host strain when added together with the bacteria in a ratio of 10 plaque‐forming units per colony‐forming unit. While we recognize the artificial laboratory conditions used for these protection assays, this work is the first to demonstrate that phages might be able protect salmonids from RTFS or CWD.

    AB - Flavobacterium psychrophilum causes rainbow trout fry syndrome (RTFS) and cold water disease (CWD) in salmonid aquaculture. We report characterization of F. psychrophilum strains and their bacteriophages isolated in Chilean salmonid aquaculture. Results suggest that under laboratory conditions phages can decrease mortality of salmonids from infection by their F. psychrophilum host strain. Twelve F. psychrophilum isolates were characterized, with DNA restriction patterns showing low diversity between strains despite their being obtained from different salmonid production sites and from different tissues. We isolated 15 bacteriophages able to infect some of the F. psychrophilum isolates and characterized six of them in detail. DNA genome sizes were close to 50 Kbp and corresponded to the Siphoviridae and Podoviridae families. One isolate, 6H, probably contains lipids as an essential virion component, based on its chloroform sensitivity and low buoyant density in CsCl. Each phage isolate rarely infected F. psychrophilum strains other than the strain used for its enrichment and isolation. Some bacteriophages could decrease mortality from intraperitoneal injection of its host strain when added together with the bacteria in a ratio of 10 plaque‐forming units per colony‐forming unit. While we recognize the artificial laboratory conditions used for these protection assays, this work is the first to demonstrate that phages might be able protect salmonids from RTFS or CWD.

    KW - Phages salmonids

    KW - Flavobacterium psychrophilum

    KW - Bacterial cold water disease

    KW - Protection

    U2 - 10.1111/j.1365-2761.2011.01336.x

    DO - 10.1111/j.1365-2761.2011.01336.x

    M3 - Journal article

    VL - 35

    SP - 193

    EP - 201

    JO - Journal of Fish Diseases

    JF - Journal of Fish Diseases

    SN - 0140-7775

    IS - 3

    ER -