Diversity and distribution of the bmp gene cluster and its Polybrominated products in the genus Pseudoalteromonas

Julia Busch, Vinayak Agarwal, Michelle Schorn, Henrique Machado, Bradley S Moore, Greg W Rouse, Lone Gram, Paul R Jensen*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The production of pentabromopseudilin and related brominated compounds by Pseudoalteromonas spp. has recently been linked to the bmp biosynthetic gene cluster. This study explored the distribution and evolutionary history of this gene cluster in the genus Pseudoalteromonas. A phylogeny of the genus revealed numerous clades that do not contain type strains, suggesting considerable species level diversity has yet to be described. Comparative genomics revealed four distinct versions of the gene cluster distributed among 19 of the 101 Pseudoalteromonas genomes examined. These were largely localized to the least inclusive clades containing the Pseudoalteromonas luteoviolacea and Pseudoalteromonas phenolica type strains and show clear evidence of gene and gene cluster loss in certain lineages. Bmp gene phylogeny is largely congruent with the Pseudoalteromonas species phylogeny, suggesting vertical inheritance within the genus. However, the gene cluster is found in three different genomic environments suggesting either chromosomal rearrangement or multiple acquisition events. Bmp conservation within certain lineages suggests the encoded products are highly relevant to the ecology of these bacteria.

Original languageEnglish
JournalEnvironmental Microbiology
Volume21
Issue number5
Pages (from-to)1575-1585
ISSN1462-2912
DOIs
Publication statusPublished - 2019

Cite this

Busch, J., Agarwal, V., Schorn, M., Machado, H., Moore, B. S., Rouse, G. W., ... Jensen, P. R. (2019). Diversity and distribution of the bmp gene cluster and its Polybrominated products in the genus Pseudoalteromonas. Environmental Microbiology, 21(5), 1575-1585 . https://doi.org/10.1111/1462-2920.14532
Busch, Julia ; Agarwal, Vinayak ; Schorn, Michelle ; Machado, Henrique ; Moore, Bradley S ; Rouse, Greg W ; Gram, Lone ; Jensen, Paul R. / Diversity and distribution of the bmp gene cluster and its Polybrominated products in the genus Pseudoalteromonas. In: Environmental Microbiology. 2019 ; Vol. 21, No. 5. pp. 1575-1585 .
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abstract = "The production of pentabromopseudilin and related brominated compounds by Pseudoalteromonas spp. has recently been linked to the bmp biosynthetic gene cluster. This study explored the distribution and evolutionary history of this gene cluster in the genus Pseudoalteromonas. A phylogeny of the genus revealed numerous clades that do not contain type strains, suggesting considerable species level diversity has yet to be described. Comparative genomics revealed four distinct versions of the gene cluster distributed among 19 of the 101 Pseudoalteromonas genomes examined. These were largely localized to the least inclusive clades containing the Pseudoalteromonas luteoviolacea and Pseudoalteromonas phenolica type strains and show clear evidence of gene and gene cluster loss in certain lineages. Bmp gene phylogeny is largely congruent with the Pseudoalteromonas species phylogeny, suggesting vertical inheritance within the genus. However, the gene cluster is found in three different genomic environments suggesting either chromosomal rearrangement or multiple acquisition events. Bmp conservation within certain lineages suggests the encoded products are highly relevant to the ecology of these bacteria.",
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Diversity and distribution of the bmp gene cluster and its Polybrominated products in the genus Pseudoalteromonas. / Busch, Julia; Agarwal, Vinayak; Schorn, Michelle; Machado, Henrique; Moore, Bradley S; Rouse, Greg W; Gram, Lone; Jensen, Paul R.

In: Environmental Microbiology, Vol. 21, No. 5, 2019, p. 1575-1585 .

Research output: Contribution to journalJournal articleResearchpeer-review

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T1 - Diversity and distribution of the bmp gene cluster and its Polybrominated products in the genus Pseudoalteromonas

AU - Busch, Julia

AU - Agarwal, Vinayak

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AU - Moore, Bradley S

AU - Rouse, Greg W

AU - Gram, Lone

AU - Jensen, Paul R

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AB - The production of pentabromopseudilin and related brominated compounds by Pseudoalteromonas spp. has recently been linked to the bmp biosynthetic gene cluster. This study explored the distribution and evolutionary history of this gene cluster in the genus Pseudoalteromonas. A phylogeny of the genus revealed numerous clades that do not contain type strains, suggesting considerable species level diversity has yet to be described. Comparative genomics revealed four distinct versions of the gene cluster distributed among 19 of the 101 Pseudoalteromonas genomes examined. These were largely localized to the least inclusive clades containing the Pseudoalteromonas luteoviolacea and Pseudoalteromonas phenolica type strains and show clear evidence of gene and gene cluster loss in certain lineages. Bmp gene phylogeny is largely congruent with the Pseudoalteromonas species phylogeny, suggesting vertical inheritance within the genus. However, the gene cluster is found in three different genomic environments suggesting either chromosomal rearrangement or multiple acquisition events. Bmp conservation within certain lineages suggests the encoded products are highly relevant to the ecology of these bacteria.

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