Disease-free monoculture farming by fungus-growing termites

Saria Otani, Victoria L. Challinor, Nina B. Kreuzenbeck, Sara Kildgaard, Søren Krath Christensen, Louise Lee Munk Larsen, Duur K. Aanen, Silas Anselm Rasmussen, Christine Beemelmanns, Michael Poulsen*

*Corresponding author for this work

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Abstract

Fungus-growing termites engage in an obligate mutualistic relationship with Termitomyces fungi, which they maintain in monocultures on specialised fungus comb structures, without apparent problems with infectious diseases. While other fungi have been reported in the symbiosis, detailed comb fungal community analyses have been lacking. Here we use culture-dependent and -independent methods to characterise fungus comb mycobiotas from three fungus-growing termite species (two genera). Internal Transcribed Spacer (ITS) gene analyses using 454 pyrosequencing and Illumina MiSeq showed that non-Termitomyces fungi were essentially absent in fungus combs, and that Termitomyces fungal crops are maintained in monocultures as heterokaryons with two or three abundant ITS variants in a single fungal strain. To explore whether the essential absence of other fungi within fungus combs is potentially due to the production of antifungal metabolites by Termitomyces or comb bacteria, we performed in vitro assays and found that both Termitomyces and chemical extracts of fungus comb material can inhibit potential fungal antagonists. Chemical analyses of fungus comb material point to a highly complex metabolome, including compounds with the potential to play roles in mediating these contaminant-free farming conditions in the termite symbiosis.
Original languageEnglish
Article number8819
JournalScientific Reports
Volume9
Issue number1
Number of pages10
ISSN2045-2322
DOIs
Publication statusPublished - 2019

Cite this

Otani, S., Challinor, V. L., Kreuzenbeck, N. B., Kildgaard, S., Krath Christensen, S., Larsen, L. L. M., ... Poulsen, M. (2019). Disease-free monoculture farming by fungus-growing termites. Scientific Reports, 9(1), [8819]. https://doi.org/10.1038/s41598-019-45364-z
Otani, Saria ; Challinor, Victoria L. ; Kreuzenbeck, Nina B. ; Kildgaard, Sara ; Krath Christensen, Søren ; Larsen, Louise Lee Munk ; Aanen, Duur K. ; Rasmussen, Silas Anselm ; Beemelmanns, Christine ; Poulsen, Michael. / Disease-free monoculture farming by fungus-growing termites. In: Scientific Reports. 2019 ; Vol. 9, No. 1.
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abstract = "Fungus-growing termites engage in an obligate mutualistic relationship with Termitomyces fungi, which they maintain in monocultures on specialised fungus comb structures, without apparent problems with infectious diseases. While other fungi have been reported in the symbiosis, detailed comb fungal community analyses have been lacking. Here we use culture-dependent and -independent methods to characterise fungus comb mycobiotas from three fungus-growing termite species (two genera). Internal Transcribed Spacer (ITS) gene analyses using 454 pyrosequencing and Illumina MiSeq showed that non-Termitomyces fungi were essentially absent in fungus combs, and that Termitomyces fungal crops are maintained in monocultures as heterokaryons with two or three abundant ITS variants in a single fungal strain. To explore whether the essential absence of other fungi within fungus combs is potentially due to the production of antifungal metabolites by Termitomyces or comb bacteria, we performed in vitro assays and found that both Termitomyces and chemical extracts of fungus comb material can inhibit potential fungal antagonists. Chemical analyses of fungus comb material point to a highly complex metabolome, including compounds with the potential to play roles in mediating these contaminant-free farming conditions in the termite symbiosis.",
author = "Saria Otani and Challinor, {Victoria L.} and Kreuzenbeck, {Nina B.} and Sara Kildgaard and {Krath Christensen}, S{\o}ren and Larsen, {Louise Lee Munk} and Aanen, {Duur K.} and Rasmussen, {Silas Anselm} and Christine Beemelmanns and Michael Poulsen",
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Otani, S, Challinor, VL, Kreuzenbeck, NB, Kildgaard, S, Krath Christensen, S, Larsen, LLM, Aanen, DK, Rasmussen, SA, Beemelmanns, C & Poulsen, M 2019, 'Disease-free monoculture farming by fungus-growing termites', Scientific Reports, vol. 9, no. 1, 8819. https://doi.org/10.1038/s41598-019-45364-z

Disease-free monoculture farming by fungus-growing termites. / Otani, Saria; Challinor, Victoria L.; Kreuzenbeck, Nina B.; Kildgaard, Sara; Krath Christensen, Søren; Larsen, Louise Lee Munk; Aanen, Duur K.; Rasmussen, Silas Anselm; Beemelmanns, Christine; Poulsen, Michael.

In: Scientific Reports, Vol. 9, No. 1, 8819, 2019.

Research output: Contribution to journalJournal articleResearchpeer-review

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T1 - Disease-free monoculture farming by fungus-growing termites

AU - Otani, Saria

AU - Challinor, Victoria L.

AU - Kreuzenbeck, Nina B.

AU - Kildgaard, Sara

AU - Krath Christensen, Søren

AU - Larsen, Louise Lee Munk

AU - Aanen, Duur K.

AU - Rasmussen, Silas Anselm

AU - Beemelmanns, Christine

AU - Poulsen, Michael

PY - 2019

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AB - Fungus-growing termites engage in an obligate mutualistic relationship with Termitomyces fungi, which they maintain in monocultures on specialised fungus comb structures, without apparent problems with infectious diseases. While other fungi have been reported in the symbiosis, detailed comb fungal community analyses have been lacking. Here we use culture-dependent and -independent methods to characterise fungus comb mycobiotas from three fungus-growing termite species (two genera). Internal Transcribed Spacer (ITS) gene analyses using 454 pyrosequencing and Illumina MiSeq showed that non-Termitomyces fungi were essentially absent in fungus combs, and that Termitomyces fungal crops are maintained in monocultures as heterokaryons with two or three abundant ITS variants in a single fungal strain. To explore whether the essential absence of other fungi within fungus combs is potentially due to the production of antifungal metabolites by Termitomyces or comb bacteria, we performed in vitro assays and found that both Termitomyces and chemical extracts of fungus comb material can inhibit potential fungal antagonists. Chemical analyses of fungus comb material point to a highly complex metabolome, including compounds with the potential to play roles in mediating these contaminant-free farming conditions in the termite symbiosis.

U2 - 10.1038/s41598-019-45364-z

DO - 10.1038/s41598-019-45364-z

M3 - Journal article

VL - 9

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 8819

ER -

Otani S, Challinor VL, Kreuzenbeck NB, Kildgaard S, Krath Christensen S, Larsen LLM et al. Disease-free monoculture farming by fungus-growing termites. Scientific Reports. 2019;9(1). 8819. https://doi.org/10.1038/s41598-019-45364-z