Discovery and description of a new serogroup 7 Streptococcus pneumoniae serotype, 7D, and structural analysis of 7C and 7D

Christian Kjeldsen, Sofie Slott, Pernille L. Elverdal, Carmen L. Sheppard, Georgia Kapatai, Norman K. Fry, Ian C. Skovsted, Jens Ø. Duus*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Streptococcus pneumoniae is characterised into 92 serotypes based on antigenic reactions of commercial rabbit sera to the capsular polysaccharides. During development of a bioinformatic serotyping tool (PneumoCaT), an isolate exhibited a novel codon at residue 385 of the glycosyltransferase gene wcwK encoding a distinct amino acid, which differentiates genogroup 7. Investigation by repeat serotyping and Quellung reaction revealed a novel pattern of factor sera with the isolate reacting very strongly with 7f, but also with 7e factor sera. The structure of the capsular polysaccharide was determined by NMR spectroscopy to be an approximately 5:1 combination of the structures of 7C and 7B, respectively, and the structure of 7C was also elucidated. All data from whole genome sequencing, NMR spectroscopy, production of antisera and serotyping of the novel 7 strain shows that it is a new serotype, which will be named in the Danish nomenclature as 7D.
Original languageEnglish
JournalCARBOHYDRATE RESEARCH
Volume463
Pages (from-to)24-31
Number of pages8
ISSN0008-6215
DOIs
Publication statusPublished - 2018

Keywords

  • NMR spectroscopy
  • Structure elucidation
  • Streptococcus pneumoniae
  • Whole genome sequence
  • Hybrid serotype

Cite this

Kjeldsen, Christian ; Slott, Sofie ; Elverdal, Pernille L. ; Sheppard, Carmen L. ; Kapatai, Georgia ; Fry, Norman K. ; Skovsted, Ian C. ; Duus, Jens Ø. / Discovery and description of a new serogroup 7 Streptococcus pneumoniae serotype, 7D, and structural analysis of 7C and 7D. In: CARBOHYDRATE RESEARCH. 2018 ; Vol. 463. pp. 24-31.
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title = "Discovery and description of a new serogroup 7 Streptococcus pneumoniae serotype, 7D, and structural analysis of 7C and 7D",
abstract = "Streptococcus pneumoniae is characterised into 92 serotypes based on antigenic reactions of commercial rabbit sera to the capsular polysaccharides. During development of a bioinformatic serotyping tool (PneumoCaT), an isolate exhibited a novel codon at residue 385 of the glycosyltransferase gene wcwK encoding a distinct amino acid, which differentiates genogroup 7. Investigation by repeat serotyping and Quellung reaction revealed a novel pattern of factor sera with the isolate reacting very strongly with 7f, but also with 7e factor sera. The structure of the capsular polysaccharide was determined by NMR spectroscopy to be an approximately 5:1 combination of the structures of 7C and 7B, respectively, and the structure of 7C was also elucidated. All data from whole genome sequencing, NMR spectroscopy, production of antisera and serotyping of the novel 7 strain shows that it is a new serotype, which will be named in the Danish nomenclature as 7D.",
keywords = "NMR spectroscopy, Structure elucidation, Streptococcus pneumoniae, Whole genome sequence, Hybrid serotype",
author = "Christian Kjeldsen and Sofie Slott and Elverdal, {Pernille L.} and Sheppard, {Carmen L.} and Georgia Kapatai and Fry, {Norman K.} and Skovsted, {Ian C.} and Duus, {Jens {\O}.}",
year = "2018",
doi = "10.1016/j.carres.2018.04.011",
language = "English",
volume = "463",
pages = "24--31",
journal = "Carbohydrate Research",
issn = "0008-6215",
publisher = "Pergamon Press",

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Discovery and description of a new serogroup 7 Streptococcus pneumoniae serotype, 7D, and structural analysis of 7C and 7D. / Kjeldsen, Christian; Slott, Sofie; Elverdal, Pernille L.; Sheppard, Carmen L.; Kapatai, Georgia; Fry, Norman K.; Skovsted, Ian C.; Duus, Jens Ø.

In: CARBOHYDRATE RESEARCH, Vol. 463, 2018, p. 24-31.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Discovery and description of a new serogroup 7 Streptococcus pneumoniae serotype, 7D, and structural analysis of 7C and 7D

AU - Kjeldsen, Christian

AU - Slott, Sofie

AU - Elverdal, Pernille L.

AU - Sheppard, Carmen L.

AU - Kapatai, Georgia

AU - Fry, Norman K.

AU - Skovsted, Ian C.

AU - Duus, Jens Ø.

PY - 2018

Y1 - 2018

N2 - Streptococcus pneumoniae is characterised into 92 serotypes based on antigenic reactions of commercial rabbit sera to the capsular polysaccharides. During development of a bioinformatic serotyping tool (PneumoCaT), an isolate exhibited a novel codon at residue 385 of the glycosyltransferase gene wcwK encoding a distinct amino acid, which differentiates genogroup 7. Investigation by repeat serotyping and Quellung reaction revealed a novel pattern of factor sera with the isolate reacting very strongly with 7f, but also with 7e factor sera. The structure of the capsular polysaccharide was determined by NMR spectroscopy to be an approximately 5:1 combination of the structures of 7C and 7B, respectively, and the structure of 7C was also elucidated. All data from whole genome sequencing, NMR spectroscopy, production of antisera and serotyping of the novel 7 strain shows that it is a new serotype, which will be named in the Danish nomenclature as 7D.

AB - Streptococcus pneumoniae is characterised into 92 serotypes based on antigenic reactions of commercial rabbit sera to the capsular polysaccharides. During development of a bioinformatic serotyping tool (PneumoCaT), an isolate exhibited a novel codon at residue 385 of the glycosyltransferase gene wcwK encoding a distinct amino acid, which differentiates genogroup 7. Investigation by repeat serotyping and Quellung reaction revealed a novel pattern of factor sera with the isolate reacting very strongly with 7f, but also with 7e factor sera. The structure of the capsular polysaccharide was determined by NMR spectroscopy to be an approximately 5:1 combination of the structures of 7C and 7B, respectively, and the structure of 7C was also elucidated. All data from whole genome sequencing, NMR spectroscopy, production of antisera and serotyping of the novel 7 strain shows that it is a new serotype, which will be named in the Danish nomenclature as 7D.

KW - NMR spectroscopy

KW - Structure elucidation

KW - Streptococcus pneumoniae

KW - Whole genome sequence

KW - Hybrid serotype

U2 - 10.1016/j.carres.2018.04.011

DO - 10.1016/j.carres.2018.04.011

M3 - Journal article

VL - 463

SP - 24

EP - 31

JO - Carbohydrate Research

JF - Carbohydrate Research

SN - 0008-6215

ER -