Directed self-organization of single DNA molecules in a nanoslit via embedded nanopit arrays

Walter Reisner, Niels Bent Larsen, Henrik K. Flyvbjerg, Jonas O. Tegenfeldt, Anders Kristensen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    We show that arrays of nanopit structures etched in a nanoslit can control the positioning and conformation of single DNA molecules in nanofluidic devices. By adjusting the spacing, organization and placement of the nanopits it is possible to immobilize DNA at predetermined regions of a device without additional chemical modification and achieve a high degree of control over local DNA conformation. DNA can be extended between two nanopits and in closely spaced arrays will self-assemble into "connect-the-dots" conformations consisting of locally pinned segments joined by fluctuating linkers. These results have broad implications for nanotechnology fields that require methods for the nanoscale positioning and manipulation of DNA.
    Original languageEnglish
    JournalProceedings of the National Academy of Science of the United States of America
    Volume106
    Issue number1
    Pages (from-to)79-84
    ISSN0027-8424
    DOIs
    Publication statusPublished - 2009

    Keywords

    • self-assembly
    • DNA conformation
    • nanofluidics
    • polymer confinement

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