TY - JOUR
T1 - Directed Evolution of CRISPR/Cas Systems for Precise Gene Editing
AU - Liu, Rongming
AU - Liang, Liya
AU - Freed, Emily F.
AU - Gill, Ryan T.
PY - 2021
Y1 - 2021
N2 - CRISPR technology is a universal tool for genome engineering that has revolutionized biotechnology. Recently identified unique CRISPR/Cas systems, as well as re-engineered Cas proteins, have rapidly expanded the functions and applications of CRISPR/Cas systems. The structures of Cas proteins are complex, containing multiple functional domains. These protein domains are evolutionarily conserved polypeptide units that generally show independent structural or functional properties. In this review, we propose using protein domains as a new way to classify protein engineering strategies for these proteins and discuss common ways to engineer key domains to modify the functions of CRISPR/Cas systems.
AB - CRISPR technology is a universal tool for genome engineering that has revolutionized biotechnology. Recently identified unique CRISPR/Cas systems, as well as re-engineered Cas proteins, have rapidly expanded the functions and applications of CRISPR/Cas systems. The structures of Cas proteins are complex, containing multiple functional domains. These protein domains are evolutionarily conserved polypeptide units that generally show independent structural or functional properties. In this review, we propose using protein domains as a new way to classify protein engineering strategies for these proteins and discuss common ways to engineer key domains to modify the functions of CRISPR/Cas systems.
U2 - 10.1016/j.tibtech.2020.07.005
DO - 10.1016/j.tibtech.2020.07.005
M3 - Journal article
C2 - 32828556
SN - 0167-7799
VL - 39
SP - 262
EP - 273
JO - Trends in Biotechnology
JF - Trends in Biotechnology
IS - 3
ER -