Direct Three-Dimensional Characterization and Multiscale Visualization of Wheat Straw Deconstruction by White Rot Fungus

Li Liu, Chen Qian, Lei Jiang, Han-Qing Yu

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Microbial degradation of lignocellulose for resource and energy recovery has received increasing interest. Despite its obvious importance, the mechanism behind the biodegradation, especially the changes of morphological structure and surface characteristics, has not been fully understood. Here, we used three-dimensional (3D) characterization and multiscale visualization methods, in combination with chemical compositional analyses, to elucidate the degradation process of wheat straw by a white rot fungus, Phanerochaete chrysosporium. It was found that the fungal attack initiated from stomata. Lignin of the straw decayed in both size and quantity, and heterogeneity in the biodegradation was observed. After treatment with the fungus, the straw surface turned from hydrophobic to hydrophilic, and the adhesion of the straw surface increased in the fungal degradation. The morphology of the straw outer layer became heterogeneous and loose with the formation of many holes with various sizes. The wasp-tunnels-like structure of the collenchyma and parenchyma of the straw as well as the fungal hyphae interspersed inside the straw structure were clearly visualized in the 3D reconstruction structure. This work offers a new insight into the mechanism of lignocellulose biodegradation and demonstrates that multiscale visualization methods could be a useful tool to explore such complex processes.
Original languageEnglish
JournalEnvironmental Science & Technology (Washington)
Volume48
Issue number16
Pages (from-to)9819-9825
Number of pages7
ISSN0013-936X
DOIs
Publication statusPublished - 2014
Externally publishedYes

Keywords

  • wheat straw deconstruction
  • white rot fungal disease
  • Fungi Plantae (Fungi, Microorganisms, Nonvascular Plants, Plants) - Basidiomycetes [15300] Phanerochaete chrysosporium species white rot fungi common strain-BKM-F-1767
  • lignin 9005-53-2
  • lignocellulose 11132-73-3
  • 10060, Biochemistry studies - General
  • 39008, Food microbiology - General and miscellaneous
  • collenchyma
  • parenchyma
  • multiscale visualization method laboratory techniques
  • optical microscopy laboratory techniques, imaging and microscopy techniques
  • scanning electron microscopy SEM laboratory techniques, diagnostic techniques, imaging and microscopy techniques
  • three-dimensional characterization mathematical and computer techniques
  • X-ray computed tomography imaging and microscopy techniques, diagnostic techniques, clinical techniques
  • X-ray photoelectron spectroscopy XPS laboratory techniques, spectrum analysis techniques
  • Bioprocess Engineering
  • Methods and Techniques
  • ENGINEERING,
  • ENVIRONMENTAL
  • RAY PHOTOELECTRON-SPECTROSCOPY
  • SOLID-STATE FERMENTATION
  • CORN STOVER
  • KRAFT PULPS
  • LIGNIN
  • SURFACE
  • DEGRADATION
  • PRETREATMENT
  • CELLULASE
  • ENZYMES

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