Direct selection of expressed sequences on a YAC clone revealed proline-rich-like genes and BARE-1 sequences physically linked to the complex ¤Mla¤ powdery mildew resistance locus of barley (¤Hordeum vulgare¤ L.)

G. Schwarz, W. Michalek, A. Jahoor, V. Mohler

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    An entire 170-kb YAC clone, spanning the complex Mla powdery mildew resistance locus of barley, was used as a hybridization probe to screen a cDNA library made from a bulk of inoculated and non-inoculated primary barley leaves for isolating expressed sequences physically linked to the resistance gene. Of 22 selected cDNA clones, six were re-located on the YAC by southern analysis. Two of these clones are predicted to encode members of the hydroxyproline-rich glycoprotein and proline-rich protein gene families which have been implicated in plant defense response. Four sequences showed high homology to the copia-like retroelement BA REI of barley, putatively involved in evolution of disease resistance loci. The high degree of clones representing barley rRNA sequences or false positives is a major disadvantage of direct selection of cDNAs in barley. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
    Original languageEnglish
    JournalPlant Science
    Volume163
    Issue number2
    Pages (from-to)307-311
    ISSN0168-9452
    DOIs
    Publication statusPublished - 2002

    Cite this

    @article{2bc4bfecba494ccc909d1f2c41871d56,
    title = "Direct selection of expressed sequences on a YAC clone revealed proline-rich-like genes and BARE-1 sequences physically linked to the complex ¤Mla¤ powdery mildew resistance locus of barley (¤Hordeum vulgare¤ L.)",
    abstract = "An entire 170-kb YAC clone, spanning the complex Mla powdery mildew resistance locus of barley, was used as a hybridization probe to screen a cDNA library made from a bulk of inoculated and non-inoculated primary barley leaves for isolating expressed sequences physically linked to the resistance gene. Of 22 selected cDNA clones, six were re-located on the YAC by southern analysis. Two of these clones are predicted to encode members of the hydroxyproline-rich glycoprotein and proline-rich protein gene families which have been implicated in plant defense response. Four sequences showed high homology to the copia-like retroelement BA REI of barley, putatively involved in evolution of disease resistance loci. The high degree of clones representing barley rRNA sequences or false positives is a major disadvantage of direct selection of cDNAs in barley. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.",
    keywords = "8-B gen",
    author = "G. Schwarz and W. Michalek and A. Jahoor and V. Mohler",
    year = "2002",
    doi = "10.1016/S0168-9452(02)00131-0",
    language = "English",
    volume = "163",
    pages = "307--311",
    journal = "Plant Science",
    issn = "0168-9452",
    publisher = "Elsevier",
    number = "2",

    }

    Direct selection of expressed sequences on a YAC clone revealed proline-rich-like genes and BARE-1 sequences physically linked to the complex ¤Mla¤ powdery mildew resistance locus of barley (¤Hordeum vulgare¤ L.). / Schwarz, G.; Michalek, W.; Jahoor, A.; Mohler, V.

    In: Plant Science, Vol. 163, No. 2, 2002, p. 307-311.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Direct selection of expressed sequences on a YAC clone revealed proline-rich-like genes and BARE-1 sequences physically linked to the complex ¤Mla¤ powdery mildew resistance locus of barley (¤Hordeum vulgare¤ L.)

    AU - Schwarz, G.

    AU - Michalek, W.

    AU - Jahoor, A.

    AU - Mohler, V.

    PY - 2002

    Y1 - 2002

    N2 - An entire 170-kb YAC clone, spanning the complex Mla powdery mildew resistance locus of barley, was used as a hybridization probe to screen a cDNA library made from a bulk of inoculated and non-inoculated primary barley leaves for isolating expressed sequences physically linked to the resistance gene. Of 22 selected cDNA clones, six were re-located on the YAC by southern analysis. Two of these clones are predicted to encode members of the hydroxyproline-rich glycoprotein and proline-rich protein gene families which have been implicated in plant defense response. Four sequences showed high homology to the copia-like retroelement BA REI of barley, putatively involved in evolution of disease resistance loci. The high degree of clones representing barley rRNA sequences or false positives is a major disadvantage of direct selection of cDNAs in barley. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

    AB - An entire 170-kb YAC clone, spanning the complex Mla powdery mildew resistance locus of barley, was used as a hybridization probe to screen a cDNA library made from a bulk of inoculated and non-inoculated primary barley leaves for isolating expressed sequences physically linked to the resistance gene. Of 22 selected cDNA clones, six were re-located on the YAC by southern analysis. Two of these clones are predicted to encode members of the hydroxyproline-rich glycoprotein and proline-rich protein gene families which have been implicated in plant defense response. Four sequences showed high homology to the copia-like retroelement BA REI of barley, putatively involved in evolution of disease resistance loci. The high degree of clones representing barley rRNA sequences or false positives is a major disadvantage of direct selection of cDNAs in barley. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

    KW - 8-B gen

    U2 - 10.1016/S0168-9452(02)00131-0

    DO - 10.1016/S0168-9452(02)00131-0

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    VL - 163

    SP - 307

    EP - 311

    JO - Plant Science

    JF - Plant Science

    SN - 0168-9452

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    ER -