Direct Quantitation on Paper Chromatograms of Radioactivity from 14c Labelled Fatty Acids

Heinz J.M. Hansen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The present investigation is based on experiments using reversed phase chromatography with horizontal development. It is shown by varying spot size and type of fatty acid that the marginal distribution throughout the thickness of the paper of the molecules to be counted renders no bias to their quantitative evaluation. The said marginal distribution, however, influences the scanning error and it can be recommended to scan low energy labelled paper chromatograms from both sides of the paper and average the results.
    Original languageEnglish
    JournalActa Chemica Scandinavica
    Volume15
    Issue number3
    Pages (from-to)670-&
    ISSN0904-213X
    Publication statusPublished - 1961

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