Direct immobilization of cholesteryl-TEG-modified oligonucleotides onto hydrophobic SU-8 surfaces

Yavuz Erkan; Ilja Czolkos; Aldo Jesorka; L. Marcus Wilhelmsson; Owe Orwar

doi:10.1021/la7005502

Direct immobilization of cholesteryl-TEG-modified oligonucleotides onto hydrophobic SU-8 surfaces

Yavuz Erkan, Ilja Czolkos, Aldo Jesorka, L. Marcus Wilhelmsson, Owe Orwar

Chalmers University of Technology

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

We introduce a rapid, simple one-step procedure for the high-yield immobilization of cholesteryl-tetraethyleneglycol-modified oligonucleotides (chol-DNA) at hydrophobic sites made of SU-8 photoresist. Topographic structures of SU-8 were microfabricated on microscope glass coverslips sputtered with a Ti/Au layer. Upon application, chol-DNA adsorbed to the SU-8 structures from solution, leaving the surrounding gold surface free of chol-DNA. chol-DNA immobilization is complete within 15 min and yields a surface coverage in the range of 20-95 pmol/cm2, which corresponds to a film density of 1012-1013 molecules/cm2. chol-DNA immobilization is stable and can be sustained despite rinsing, drying, dry storage for several hours, and rehydration of chips. Furthermore, complementary DNA in solution hybridizes efficiently to immobilized chol-DNA. © 2007 American Chemical Society.
Keyword: Chemical modification

Original language	English
Journal	Langmuir
Volume	23
Issue number	10
Pages (from-to)	5259-5263
ISSN	0743-7463
DOIs	https://doi.org/10.1021/la7005502
Publication status	Published - 2007
Externally published	Yes

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title = "Direct immobilization of cholesteryl-TEG-modified oligonucleotides onto hydrophobic SU-8 surfaces",

abstract = "We introduce a rapid, simple one-step procedure for the high-yield immobilization of cholesteryl-tetraethyleneglycol-modified oligonucleotides (chol-DNA) at hydrophobic sites made of SU-8 photoresist. Topographic structures of SU-8 were microfabricated on microscope glass coverslips sputtered with a Ti/Au layer. Upon application, chol-DNA adsorbed to the SU-8 structures from solution, leaving the surrounding gold surface free of chol-DNA. chol-DNA immobilization is complete within 15 min and yields a surface coverage in the range of 20-95 pmol/cm2, which corresponds to a film density of 1012-1013 molecules/cm2. chol-DNA immobilization is stable and can be sustained despite rinsing, drying, dry storage for several hours, and rehydration of chips. Furthermore, complementary DNA in solution hybridizes efficiently to immobilized chol-DNA. {\textcopyright} 2007 American Chemical Society. Keyword: Chemical modification",

author = "Yavuz Erkan and Ilja Czolkos and Aldo Jesorka and Wilhelmsson, {L. Marcus} and Owe Orwar",

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volume = "23",

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T1 - Direct immobilization of cholesteryl-TEG-modified oligonucleotides onto hydrophobic SU-8 surfaces

AU - Erkan, Yavuz

AU - Czolkos, Ilja

AU - Jesorka, Aldo

AU - Wilhelmsson, L. Marcus

AU - Orwar, Owe

PY - 2007

Y1 - 2007

N2 - We introduce a rapid, simple one-step procedure for the high-yield immobilization of cholesteryl-tetraethyleneglycol-modified oligonucleotides (chol-DNA) at hydrophobic sites made of SU-8 photoresist. Topographic structures of SU-8 were microfabricated on microscope glass coverslips sputtered with a Ti/Au layer. Upon application, chol-DNA adsorbed to the SU-8 structures from solution, leaving the surrounding gold surface free of chol-DNA. chol-DNA immobilization is complete within 15 min and yields a surface coverage in the range of 20-95 pmol/cm2, which corresponds to a film density of 1012-1013 molecules/cm2. chol-DNA immobilization is stable and can be sustained despite rinsing, drying, dry storage for several hours, and rehydration of chips. Furthermore, complementary DNA in solution hybridizes efficiently to immobilized chol-DNA. © 2007 American Chemical Society. Keyword: Chemical modification

AB - We introduce a rapid, simple one-step procedure for the high-yield immobilization of cholesteryl-tetraethyleneglycol-modified oligonucleotides (chol-DNA) at hydrophobic sites made of SU-8 photoresist. Topographic structures of SU-8 were microfabricated on microscope glass coverslips sputtered with a Ti/Au layer. Upon application, chol-DNA adsorbed to the SU-8 structures from solution, leaving the surrounding gold surface free of chol-DNA. chol-DNA immobilization is complete within 15 min and yields a surface coverage in the range of 20-95 pmol/cm2, which corresponds to a film density of 1012-1013 molecules/cm2. chol-DNA immobilization is stable and can be sustained despite rinsing, drying, dry storage for several hours, and rehydration of chips. Furthermore, complementary DNA in solution hybridizes efficiently to immobilized chol-DNA. © 2007 American Chemical Society. Keyword: Chemical modification

U2 - 10.1021/la7005502

DO - 10.1021/la7005502

M3 - Journal article

C2 - 17432889

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JO - Langmuir

JF - Langmuir

IS - 10

ER -

Direct immobilization of cholesteryl-TEG-modified oligonucleotides onto hydrophobic SU-8 surfaces

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