Direct detection of candida albicans with a membrane based electrochemical impedance spectroscopy sensor

Dorota Kwasny, Sheida Esmail Tehrani, Catarina Almeida, Ida Schjødt, Maria Dimaki, Winnie Edith Svendsen*

*Corresponding author for this work

    Research output: Contribution to journalJournal articleResearchpeer-review

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    Abstract

    Candidemia and invasive candidiasis is a cause of high mortality and morbidity rates among hospitalized patients worldwide. The occurrence of the infections increases due to the complexity of the patients and overuse of the antifungal therapy. The current Candida detection method includes blood culturing which is a lengthy procedure and thus delays the administration of the antifungal therapy. Even though the results are available after 48 h it is still the gold standard in pathogen detection in a hospital setting. In this work we present an electrochemical impedance sensor that is capable of detecting Candida albicans yeast. The yeast cells are captured on electrodes specifically functionalized with anti-Candida antibodies and detection is achieved by electrochemical impedance spectroscopy. The sensor allows for detection of the yeast cells at clinically relevant concentrations in less than 1 h.
    Original languageEnglish
    Article number2214
    JournalSensors
    Volume18
    Issue number7
    ISSN1424-8220
    DOIs
    Publication statusPublished - 2018

    Keywords

    • Candida
    • Electrochemical impedance spectroscopy
    • Membrane based sensor

    Cite this

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    title = "Direct detection of candida albicans with a membrane based electrochemical impedance spectroscopy sensor",
    abstract = "Candidemia and invasive candidiasis is a cause of high mortality and morbidity rates among hospitalized patients worldwide. The occurrence of the infections increases due to the complexity of the patients and overuse of the antifungal therapy. The current Candida detection method includes blood culturing which is a lengthy procedure and thus delays the administration of the antifungal therapy. Even though the results are available after 48 h it is still the gold standard in pathogen detection in a hospital setting. In this work we present an electrochemical impedance sensor that is capable of detecting Candida albicans yeast. The yeast cells are captured on electrodes specifically functionalized with anti-Candida antibodies and detection is achieved by electrochemical impedance spectroscopy. The sensor allows for detection of the yeast cells at clinically relevant concentrations in less than 1 h.",
    keywords = "Candida, Electrochemical impedance spectroscopy, Membrane based sensor",
    author = "Dorota Kwasny and {Esmail Tehrani}, Sheida and Catarina Almeida and Ida Schj{\o}dt and Maria Dimaki and Svendsen, {Winnie Edith}",
    year = "2018",
    doi = "10.3390/s18072214",
    language = "English",
    volume = "18",
    journal = "Sensors",
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    publisher = "M D P I AG",
    number = "7",

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    Direct detection of candida albicans with a membrane based electrochemical impedance spectroscopy sensor. / Kwasny, Dorota; Esmail Tehrani, Sheida; Almeida, Catarina; Schjødt, Ida; Dimaki, Maria; Svendsen, Winnie Edith.

    In: Sensors, Vol. 18, No. 7, 2214, 2018.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Direct detection of candida albicans with a membrane based electrochemical impedance spectroscopy sensor

    AU - Kwasny, Dorota

    AU - Esmail Tehrani, Sheida

    AU - Almeida, Catarina

    AU - Schjødt, Ida

    AU - Dimaki, Maria

    AU - Svendsen, Winnie Edith

    PY - 2018

    Y1 - 2018

    N2 - Candidemia and invasive candidiasis is a cause of high mortality and morbidity rates among hospitalized patients worldwide. The occurrence of the infections increases due to the complexity of the patients and overuse of the antifungal therapy. The current Candida detection method includes blood culturing which is a lengthy procedure and thus delays the administration of the antifungal therapy. Even though the results are available after 48 h it is still the gold standard in pathogen detection in a hospital setting. In this work we present an electrochemical impedance sensor that is capable of detecting Candida albicans yeast. The yeast cells are captured on electrodes specifically functionalized with anti-Candida antibodies and detection is achieved by electrochemical impedance spectroscopy. The sensor allows for detection of the yeast cells at clinically relevant concentrations in less than 1 h.

    AB - Candidemia and invasive candidiasis is a cause of high mortality and morbidity rates among hospitalized patients worldwide. The occurrence of the infections increases due to the complexity of the patients and overuse of the antifungal therapy. The current Candida detection method includes blood culturing which is a lengthy procedure and thus delays the administration of the antifungal therapy. Even though the results are available after 48 h it is still the gold standard in pathogen detection in a hospital setting. In this work we present an electrochemical impedance sensor that is capable of detecting Candida albicans yeast. The yeast cells are captured on electrodes specifically functionalized with anti-Candida antibodies and detection is achieved by electrochemical impedance spectroscopy. The sensor allows for detection of the yeast cells at clinically relevant concentrations in less than 1 h.

    KW - Candida

    KW - Electrochemical impedance spectroscopy

    KW - Membrane based sensor

    U2 - 10.3390/s18072214

    DO - 10.3390/s18072214

    M3 - Journal article

    VL - 18

    JO - Sensors

    JF - Sensors

    SN - 1424-8220

    IS - 7

    M1 - 2214

    ER -