Direct comparison of leaf plasmodesma structure and function in relation to phloem loading type

Johannes Liesche, Chen Gao, Piotr Binczycki, Signe Randi Andersen, Hanna Rademaker, Alexander Schulz*, Helle Juel Martens

*Corresponding author for this work

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Abstract

The export of photosynthetically produced sugars from leaves depends on plasmodesmatal transport of sugar molecules from mesophyll to phloem. Traditionally, the density of plasmodesmata (PD) along this phloem loading pathway has been used as a defining feature of different phloem loading types, with species proposed to have either many or few PD between the phloem and surrounding cells of the leaf. However, quantitative determination of PD density has rarely been performed. Moreover, the structure of PD has not been considered, even though it could impact permeability, and functional data is only available for very few species. Here, a comparison of PD density, structure and function using data from transmission electron microscopy and live-cell microscopy was conducted for all relevant cell-cell interfaces in leaves of nine species. These species represent the three principal phloem loading types currently discussed in literature. Results show that relative PD density among the different cell-cell interfaces in one species, but not absolute PD density, is indicative of phloem loading type. PD density data of single interfaces, even combined with PD diameter and length data, did not correlate with the intercellular diffusion capacity measured by the fluorescence loss in photobleaching method. This means that PD substructure not visible on standard transmission electron micrographs may have a strong influence on permeability. Furthermore, the results support a proposed passive symplasmic loading mechanism in the tree species horse-chestnut (Aesculus hippocastanum), birch (Betula pubescens), apple (Malus domestica) and poplar (Populus x canescens) as functional cell coupling and PD structure differed from active symplasmic and apoplasmic phloem loading species.
Original languageEnglish
JournalPlant Physiology
Volume179
Issue number4
Pages (from-to)1768-1778
Number of pages11
ISSN0032-0889
DOIs
Publication statusPublished - 2019

Cite this

Liesche, J., Gao, C., Binczycki, P., Andersen, S. R., Rademaker, H., Schulz, A., & Martens, H. J. (2019). Direct comparison of leaf plasmodesma structure and function in relation to phloem loading type. Plant Physiology, 179(4), 1768-1778. https://doi.org/10.1104/pp.18.01353