Differentiation of five strains of infectious bursal disease virus: Development of a strain-specific multiplex PCR

M. Kusk, Susanne Kabell, Poul Henrik Jørgensen, K. J. Handberg

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Infectious bursal disease virus (IBDV) is a major cause of disease problems in the poultry industry and vaccination has therefore been applied intensively to control the infection. The classical methods of detection and characterization of IBDV are by the use of immunodiffusion test and histopathology. Since these methods are laborious and have low specificity alternatives are needed. In the present study, we report the development of a strain-specific multiplex RT-PCR technique, which can detect and differentiate between field strains of IBDV and vaccine virus strains including a so-called hot vaccine strain widely used in the European poultry industry. The method, which is highly specific, fast and inexpensive, can be applied in all laboratories with basal PCR capabilities and equipment.
    Original languageEnglish
    JournalVeterinary Microbiology
    Volume109
    Issue number3-4
    Pages (from-to)159-167
    ISSN0378-1135
    Publication statusPublished - 2005

    Keywords

    • vvIBDV
    • RT-PCR
    • multiplex PCR
    • virus differentiation

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