Differential serodiagnostics of Toxocara canis and Toxocara cati - is it possible?

Casper Sahl Poulsen, S. Skov, A. Yoshida, P. Skallerup, H. Maruyama, S. M. Thamsborg, P. Nejsum

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

One of the most common zoonotic helminth infections is caused by species in the genus Toxocara, particularly Toxocara canis and T.cati (Syn. T.mystax). However, their relative contribution to toxocarosis in humans remains largely unknown because causative larvae are seldom recovered and uncertainties regarding the validity of existing serological assays. In this study, we used sera from a pig model experimentally infected with T.canis and T.cati to evaluate whether a Western blot could discriminate between the two species. No proteins were observed that could be used as a diagnostic tool. In addition, a heterogenic protein pattern between individual hosts was found, which was most pronounced in the T.cati-infected pigs. There is therefore an urgent need to optimize and validate current methods or develop new species-specific serological methods in order to implement appropriate control measures.
Original languageEnglish
JournalParasite Immunology
Volume37
Issue number4
Pages (from-to)204-207
Number of pages4
ISSN0141-9838
DOIs
Publication statusPublished - 2015
Externally publishedYes

Keywords

  • IMMUNOLOGY
  • PARASITOLOGY
  • VISCERAL LARVA MIGRANS
  • CLINICAL EXPRESSION
  • ANTIGEN
  • IMMUNODIAGNOSIS
  • EPIDEMIOLOGY
  • INFECTION
  • DIAGNOSIS
  • HUMANS
  • PIGS
  • diagnosis
  • Toxocara canis
  • Toxocara cati
  • Western blot
  • zoonosis
  • Animals
  • Antigens, Helminth
  • Blotting, Western
  • Cross Reactions
  • Diagnosis, Differential
  • Disease Models, Animal
  • Humans
  • Species Specificity
  • Specific Pathogen-Free Organisms
  • Sus scrofa
  • Toxocara
  • Toxocariasis
  • Zoonoses
  • toxocariasis Toxocariasis (MeSH) parasitic disease diagnosis
  • Artiodactyla Mammalia Vertebrata Chordata Animalia (Animals, Artiodactyls, Chordates, Mammals, Nonhuman Vertebrates, Nonhuman Mammals, Vertebrates) - Suidae [85740] pig common host male breed-Duroc, breed-Danish Landrace, breed-Yorkshire
  • Aschelminthes Helminthes Invertebrata Animalia (Animals, Aschelminths, Helminths, Invertebrates) - Nematoda [51300] Toxocara canis species parasite Toxocara cati species parasite
  • 12504, Pathology - Diagnostic
  • 15002, Blood - Blood and lymph studies
  • 15004, Blood - Blood cell studies
  • 38002, Veterinary science - General and methods
  • 60502, Parasitology - General
  • 60506, Parasitology - Veterinary
  • 64016, Invertebrata: comparative, experimental morphology, physiology and pathology - Aschelminthes
  • Medical Sciences
  • serum blood and lymphatics
  • differential serodiagnostic method clinical techniques, diagnostic techniques
  • Methods and Techniques
  • Parasitology
  • Veterinary Medicine

Fingerprint Dive into the research topics of 'Differential serodiagnostics of Toxocara canis and Toxocara cati - is it possible?'. Together they form a unique fingerprint.

Cite this