Differential Plasma MicroRNA Profiles in HBeAg Positive and HBeAg Negative Children with Chronic Hepatitis B

Thilde Nordmann Winther, Claus Heiner Bang-Berthelsen, Ida Louise Heiberg, Flemming Pociot, Birthe Hogh

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Abstract

Background and Aim: Children chronically infected with hepatitis B virus (HBV) are at high risk of progressive liver disease. However, no treatment is available that is consistently effective in curing chronic hepatitis B (CHB) in children. Improved understanding of the natural course of disease is warranted. Identification of specific microRNA (miRNA) profiles in children chronically infected with HBV may provide insight into the pathogenesis of CHB and lead to advances in the management of children with CHB.Patients and Methods: MiRNA PCR panels were employed to screen plasma levels of 739 miRNAs in pooled samples from HBeAg positive, HBeAg negative, and healthy children. The three groups' plasma miRNA profiles were compared, and aberrantly expressed miRNAs were identified. The identified miRNAs were then validated. Individual RT-qPCRs were performed on plasma from 34 HBeAg positive, 26 HBeAg negative, and 60 healthy children.Results: A panel of 16 plasma miRNAs were identified as aberrantly expressed in HBeAg positive and HBeAg negative children (p
Original languageEnglish
Article numbere58236
JournalP L o S One
Volume8
Issue number3
Number of pages10
ISSN1932-6203
DOIs
Publication statusPublished - 2013
Externally publishedYes

Keywords

  • Agricultural and Biological Sciences (all)
  • Biochemistry, Genetics and Molecular Biology (all)
  • Medicine (all)
  • alanine aminotransferase
  • hepatitis B(e) antigen
  • microRNA
  • virus DNA
  • virus RNA
  • alanine aminotransferase blood level
  • article
  • child
  • controlled study
  • disease course
  • female
  • hepatitis B
  • Hepatitis B virus
  • human
  • major clinical study
  • male
  • pathogenesis
  • reverse transcription polymerase chain reaction
  • RNA extraction
  • upregulation
  • virus load
  • Adolescent
  • Case-Control Studies
  • Child
  • Child, Preschool
  • DNA, Viral
  • Female
  • Hepatitis B e Antigens
  • Hepatitis B, Chronic
  • Humans
  • Infant
  • Liver
  • Male
  • MicroRNAs
  • Polymerase Chain Reaction
  • MULTIDISCIPLINARY
  • E-ANTIGEN SEROCONVERSION
  • VIRUS-INFECTION
  • HEPATOCELLULAR-CARCINOMA
  • NATURAL-HISTORY
  • SURFACE-ANTIGEN
  • IMMUNE-SYSTEM
  • EXPRESSION
  • CELLS
  • MANAGEMENT
  • CLEARANCE
  • Liver Diseases
  • Genetics - General
  • Genetics - Human
  • Biochemistry studies - Nucleic acids, purines and pyrimidines
  • Pathology - General
  • Digestive system - Pathology
  • Blood - Blood and lymph studies
  • Blood - Blood cell studies
  • Pediatrics
  • Genetics of bacteria and viruses
  • Virology - General and methods
  • Immunology - Immunopathology, tissue immunology
  • Medical and clinical microbiology - Virology
  • DNA and RNA Reverse Transcribing Viruses, Microorganisms, Viruses
  • Animals, Chordates, Humans, Mammals, Primates, Vertebrates
  • clinical parameter
  • virological parameter
  • immunological stage

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