Differential expression of small RNAs under chemical stress and fed-batch fermentation in E. coli

Martin Holm Rau; Alex Toftgaard Nielsen; Katherine Long

doi:10.1186/s12864-015-2231-8

Differential expression of small RNAs under chemical stress and fed-batch fermentation in E. coli

Martin Holm Rau, Alex Toftgaard Nielsen, Katherine Long

Novo Nordisk Foundation Center for Biosustainability

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Abstract

Bacterial small RNAs (sRNAs) are recognized as posttranscriptional regulators involved in the control of bacterial lifestyle and adaptation to stressful conditions. Although chemical stress due to the toxicity of precursor and product compounds is frequently encountered in microbial bioprocessing applications, the involvement of sRNAs in this process is not well understood. We have used RNA sequencing to map sRNA expression in E. coli under chemical stress and high cell density fermentation conditions with the aim of identifying sRNAs involved in the transcriptional response and those with potential roles in stress tolerance.

Original language	English
Journal	B M C Genomics
Volume	16
Issue number	1051
Number of pages	16
ISSN	1471-2164
DOIs	https://doi.org/10.1186/s12864-015-2231-8
Publication status	Published - 2015

Keywords

sRNA
RNA-seq
Fermentation
Chemical stress
MicF
RybB
OmrB
CyaR
RyhB

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10.1186/s12864-015-2231-8

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BacTory: Biorefinery Training Platform: Bacterial Factories for Sustainable Chemical and Drug Production
Molin, S., Nørholm, M., Cavaleiro, M., Machado, H., Wendel, S., van der Helm, E., Bojanovic, K., Vazquez Albacete, D., D'Arrigo, I., Li, S., Yang, X., Redl, S. M. A., Pogrebnyakov, I., Lehning, C. E., Giubergia, S., Calero Valdayo, P. M. & Lohmann, R.
01/10/2012 → 31/01/2017
Project: Research

Cite this

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title = "Differential expression of small RNAs under chemical stress and fed-batch fermentation in E. coli",

abstract = "Bacterial small RNAs (sRNAs) are recognized as posttranscriptional regulators involved in the control of bacterial lifestyle and adaptation to stressful conditions. Although chemical stress due to the toxicity of precursor and product compounds is frequently encountered in microbial bioprocessing applications, the involvement of sRNAs in this process is not well understood. We have used RNA sequencing to map sRNA expression in E. coli under chemical stress and high cell density fermentation conditions with the aim of identifying sRNAs involved in the transcriptional response and those with potential roles in stress tolerance.",

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T1 - Differential expression of small RNAs under chemical stress and fed-batch fermentation in E. coli

AU - Rau, Martin Holm

AU - Nielsen, Alex Toftgaard

AU - Long, Katherine

PY - 2015

Y1 - 2015

N2 - Bacterial small RNAs (sRNAs) are recognized as posttranscriptional regulators involved in the control of bacterial lifestyle and adaptation to stressful conditions. Although chemical stress due to the toxicity of precursor and product compounds is frequently encountered in microbial bioprocessing applications, the involvement of sRNAs in this process is not well understood. We have used RNA sequencing to map sRNA expression in E. coli under chemical stress and high cell density fermentation conditions with the aim of identifying sRNAs involved in the transcriptional response and those with potential roles in stress tolerance.

AB - Bacterial small RNAs (sRNAs) are recognized as posttranscriptional regulators involved in the control of bacterial lifestyle and adaptation to stressful conditions. Although chemical stress due to the toxicity of precursor and product compounds is frequently encountered in microbial bioprocessing applications, the involvement of sRNAs in this process is not well understood. We have used RNA sequencing to map sRNA expression in E. coli under chemical stress and high cell density fermentation conditions with the aim of identifying sRNAs involved in the transcriptional response and those with potential roles in stress tolerance.

KW - sRNA

KW - RNA-seq

KW - Fermentation

KW - Chemical stress

KW - MicF

KW - RybB

KW - OmrB

KW - CyaR

KW - RyhB

U2 - 10.1186/s12864-015-2231-8

DO - 10.1186/s12864-015-2231-8

M3 - Journal article

C2 - 26653712

SN - 1471-2164

VL - 16

JO - B M C Genomics

JF - B M C Genomics

IS - 1051

ER -

Differential expression of small RNAs under chemical stress and fed-batch fermentation in E. coli

Abstract

Keywords

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Projects

BacTory: Biorefinery Training Platform: Bacterial Factories for Sustainable Chemical and Drug Production

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