Differential expression of small RNAs under chemical stress and fed-batch fermentation in E. coli

Martin Holm Rau, Alex Toftgaard Nielsen, Katherine Long

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Abstract

Bacterial small RNAs (sRNAs) are recognized as posttranscriptional regulators involved in the control of bacterial lifestyle and adaptation to stressful conditions. Although chemical stress due to the toxicity of precursor and product compounds is frequently encountered in microbial bioprocessing applications, the involvement of sRNAs in this process is not well understood. We have used RNA sequencing to map sRNA expression in E. coli under chemical stress and high cell density fermentation conditions with the aim of identifying sRNAs involved in the transcriptional response and those with potential roles in stress tolerance.
Original languageEnglish
JournalB M C Genomics
Volume16
Issue number1051
Number of pages16
ISSN1471-2164
DOIs
Publication statusPublished - 2015

Keywords

  • sRNA
  • RNA-seq
  • Fermentation
  • Chemical stress
  • MicF
  • RybB
  • OmrB
  • CyaR
  • RyhB

Projects

BacTory: Biorefinery Training Platform: Bacterial Factories for Sustainable Chemical and Drug Production

Molin, S., Nørholm, M., Cavaleiro, M., Machado, H., Wendel, S., van der Helm, E., Bojanovic, K., Vazquez Albacete, D., D'Arrigo, I., Li, S., Yang, X., Redl, S. M. A., Pogrebnyakov, I., Lehning, C. E., Giubergia, S., Calero Valdayo, P. M. & Lohmann, R.

01/10/201231/01/2017

Project: Research

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