Differences in inflammation and acute phase response but similar genotoxicity in mice following pulmonary exposure to graphene oxide and reduced graphene oxide

Research output: Contribution to journalJournal article – Annual report year: 2017Researchpeer-review

Documents

DOI

  • Author: Bengtson, Stefan

    National Research Centre for the Working Environment, Denmark

  • Author: Knudsen, Kristina Bram

    National Research Centre for the Working Environment, Denmark

  • Author: Kyjovska, Zdenka O.

    National Research Centre for the Working Environment, Denmark

  • Author: Berthing, Trine

    National Research Centre for the Working Environment, Denmark

  • Author: Skaug, Vidar

    National Institute of Occupational Health, Norway

  • Author: Levin, Marcus

    National Research Centre for the Working Environment, Denmark

  • Author: Koponen, Ismo K.

    National Research Centre for the Working Environment, Denmark

  • Author: Shivayogimath, Abhay

    Nanocarbon, Department of Micro- and Nanotechnology, Technical University of Denmark, Ørsteds Plads, 2800, Kgs. Lyngby, Denmark

  • Author: Booth, Tim

    Nanocarbon, Department of Micro- and Nanotechnology, Technical University of Denmark, Ørsteds Plads, 2800, Kgs. Lyngby, Denmark

  • Author: Alonso, Beatriz

    Graphenea, Spain

  • Author: Pesquera, Amaia

    Graphenea, Spain

  • Author: Zurutuza, Amaia

    Graphenea, Spain

  • Author: Thomsen, Birthe L.

    National Research Centre for the Working Environment, Denmark

  • Author: Troelsen, Jesper T.

    Roskilde University, Denmark

  • Author: Jacobsen, Nicklas R.

    National Research Centre for the Working Environment, Denmark

  • Author: Vogel, Ulla Birgitte

    National Research Centre for the Working Environment, Denmark

View graph of relations

We investigated toxicity of 2-3 layered >1 μm sized graphene oxide (GO) and reduced graphene oxide (rGO) in mice following single intratracheal exposure with respect to pulmonary inflammation, acute phase response (biomarker for risk of cardiovascular disease) and genotoxicity. In addition, we assessed exposure levels of particulate matter emitted during production of graphene in a clean room and in a normal industrial environment using chemical vapour deposition. Toxicity was evaluated at day 1, 3, 28 and 90 days (18, 54 and 162 μg/mouse), except for GO exposed mice at day 28 and 90 where only the lowest dose was evaluated. GO induced a strong acute inflammatory response together with a pulmonary (Serum-Amyloid A, Saa3) and hepatic (Saa1) acute phase response. rGO induced less acute, but a constant and prolonged inflammation up to day 90. Lung histopathology showed particle agglomerates at day 90 without signs of fibrosis. In addition, DNA damage in BAL cells was observed across time points and doses for both GO and rGO. In conclusion, pulmonary exposure to GO and rGO induced inflammation, acute phase response and genotoxicity but no fibrosis.
Original languageEnglish
JournalP L o S One
Volume12
Issue number6
Pages (from-to)e0178355
Number of pages25
ISSN1932-6203
DOIs
Publication statusPublished - 2017
CitationsWeb of Science® Times Cited: No match on DOI

Download statistics

No data available

ID: 133054340