Dietary Tools To Modulate Glycogen Storage in Gilthead Seabream Muscle: Glycerol Supplementation

Tomé S. Silva, Elisabete Matos, Odete D. Cordeiro, Rita Colen, Tune Wulff, Eduardo Sampaio, Vera Sousa, Luisa M. P. Valente, Amparo Gonçalves, Joana M. G. Silva, Narcisa Bandarra, Maria Leonor Nunes, Maria Teresa Dinis, Jorge Dias, Flemming Jessen, Pedro M. Rodrigues

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The quality and shelf life of fish meat products depend on the skeletal muscle’s energetic state at slaughter, as meat decomposition processes can be exacerbated by energy depletion. In this study, we tested dietary glycerol as a way of replenishing muscle glycogen reserves of farmed gilthead seabream. Two diets were tested in duplicate (n = 42/tank). Results show 5% inclusion of crude glycerol in gilthead seabream diets induces increased muscle glycogen, ATP levels and firmness, with no deleterious effects in terms of growth, proximate composition, fatty acid profile, oxidative state, and organoleptic properties (aroma and color). Proteomic analysis showed a low impact of glycerol-supplementation on muscle metabolism, with most changes probably reflecting increased stress coping capacity in glycerol-fed fish. This suggests inclusion of crude glycerol in gilthead seabream diets (particularly in the finishing phase) seems like a viable strategy to increase glycogen deposition in muscle without negatively impacting fish welfare and quality.
Original languageEnglish
JournalJournal of Agricultural and Food Chemistry
Volume60
Issue number42
Pages (from-to)10613–10624
ISSN0021-8561
DOIs
Publication statusPublished - 2012

Keywords

  • crude glycerol
  • muscle glycogen
  • gilthead seabream
  • flesh quality
  • fish proteomics
  • aquaculture

Cite this

Silva, T. S., Matos, E., Cordeiro, O. D., Colen, R., Wulff, T., Sampaio, E., Sousa, V., Valente, L. M. P., Gonçalves, A., Silva, J. M. G., Bandarra, N., Nunes, M. L., Dinis, M. T., Dias, J., Jessen, F., & Rodrigues, P. M. (2012). Dietary Tools To Modulate Glycogen Storage in Gilthead Seabream Muscle: Glycerol Supplementation. Journal of Agricultural and Food Chemistry, 60(42), 10613–10624. https://doi.org/10.1021/jf3023244