Dietary methionine level affects growth performance and hepatic gene expression of GH-IGF system and protein turnover regulators in rainbow trout (Oncorhynchus mykiss) fed plant protein-based diets

Marine Rolland, Anne Johanne Tang Dalsgaard, Jorgen Holm, Pedro Gomez-Requeni, Peter Vilhelm Skov

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The effects of dietary level of methionine were investigated in juvenile rainbow trout (Oncorhynchus mykiss) fed five plant-based diets containing increasing content of crystalline methionine (Met), in a six week growth trial. Changes in the hepatic expression of genes related to i) the somatotropic axis: including the growth hormone receptor I (GHR-I), insulin-like growth hormones land II (IGF-I and IGF-II, respectively), and insulin-like growth hormone binding protein-1b (IGFBP-1b); and ii) protein turnover: including the target of rapamycin protein (TOR), proteasome 20 delta (Prot 20D), cathepsin L, calpains 1 and 2 (Capn 1 and Capn 2, respectively), and calpastatin long and short isoforms (CAST-L and CAST-S, respectively) were measured for each dietary treatment. The transcript levels of GHR-I and IGF-I increased linearly with the increase of dietary Met content (P <0.01), reflecting overall growth performances. The apparent capacity for hepatic protein degradation (derived from the gene expression of TOR, Prot 20D, Capn 1, Capn 2, CAST-L and CAST-S) decreased with increasing dietary Met level in a relatively linear manner. Our results suggest that Met availability affects, directly or indirectly, the expression of genes involved in the GH/IGF axis response and protein turnover, which are centrally involved in the regulation of growth. (C) 2014 Elsevier Inc. All rights reserved.
Original languageEnglish
JournalComparative Biochemistry and Physiology - Part B: Biochemistry & Molecular Biology
Volume181
Pages (from-to)33-41
ISSN1096-4959
DOIs
Publication statusPublished - 2015

Keywords

  • BIOCHEMISTRY
  • ZOOLOGY
  • BREAM SPARUS-AURATA
  • MESSENGER-RNA EXPRESSION
  • FACTOR-BINDING PROTEIN-1
  • AMINO-ACID-CONCENTRATIONS
  • FACTOR-I
  • DIFFERENTIAL EXPRESSION
  • NITROGEN-UTILIZATION
  • SOMATOTROPIC AXIS
  • ATLANTIC SALMON
  • MUSCLE GROWTH
  • Fish
  • Crystalline amino acid
  • Somatotropic axis
  • Proteolytic pathways
  • TOR

Cite this

@article{14b68e636a1746988dcf6c8036688768,
title = "Dietary methionine level affects growth performance and hepatic gene expression of GH-IGF system and protein turnover regulators in rainbow trout (Oncorhynchus mykiss) fed plant protein-based diets",
abstract = "The effects of dietary level of methionine were investigated in juvenile rainbow trout (Oncorhynchus mykiss) fed five plant-based diets containing increasing content of crystalline methionine (Met), in a six week growth trial. Changes in the hepatic expression of genes related to i) the somatotropic axis: including the growth hormone receptor I (GHR-I), insulin-like growth hormones land II (IGF-I and IGF-II, respectively), and insulin-like growth hormone binding protein-1b (IGFBP-1b); and ii) protein turnover: including the target of rapamycin protein (TOR), proteasome 20 delta (Prot 20D), cathepsin L, calpains 1 and 2 (Capn 1 and Capn 2, respectively), and calpastatin long and short isoforms (CAST-L and CAST-S, respectively) were measured for each dietary treatment. The transcript levels of GHR-I and IGF-I increased linearly with the increase of dietary Met content (P <0.01), reflecting overall growth performances. The apparent capacity for hepatic protein degradation (derived from the gene expression of TOR, Prot 20D, Capn 1, Capn 2, CAST-L and CAST-S) decreased with increasing dietary Met level in a relatively linear manner. Our results suggest that Met availability affects, directly or indirectly, the expression of genes involved in the GH/IGF axis response and protein turnover, which are centrally involved in the regulation of growth. (C) 2014 Elsevier Inc. All rights reserved.",
keywords = "BIOCHEMISTRY, ZOOLOGY, BREAM SPARUS-AURATA, MESSENGER-RNA EXPRESSION, FACTOR-BINDING PROTEIN-1, AMINO-ACID-CONCENTRATIONS, FACTOR-I, DIFFERENTIAL EXPRESSION, NITROGEN-UTILIZATION, SOMATOTROPIC AXIS, ATLANTIC SALMON, MUSCLE GROWTH, Fish, Crystalline amino acid, Somatotropic axis, Proteolytic pathways, TOR",
author = "Marine Rolland and Dalsgaard, {Anne Johanne Tang} and Jorgen Holm and Pedro Gomez-Requeni and Skov, {Peter Vilhelm}",
year = "2015",
doi = "10.1016/j.cbpb.2014.11.009",
language = "English",
volume = "181",
pages = "33--41",
journal = "Comparative Biochemistry and Physiology - Part B: Biochemistry & Molecular Biology",
issn = "1096-4959",
publisher = "Elsevier",

}

TY - JOUR

T1 - Dietary methionine level affects growth performance and hepatic gene expression of GH-IGF system and protein turnover regulators in rainbow trout (Oncorhynchus mykiss) fed plant protein-based diets

AU - Rolland, Marine

AU - Dalsgaard, Anne Johanne Tang

AU - Holm, Jorgen

AU - Gomez-Requeni, Pedro

AU - Skov, Peter Vilhelm

PY - 2015

Y1 - 2015

N2 - The effects of dietary level of methionine were investigated in juvenile rainbow trout (Oncorhynchus mykiss) fed five plant-based diets containing increasing content of crystalline methionine (Met), in a six week growth trial. Changes in the hepatic expression of genes related to i) the somatotropic axis: including the growth hormone receptor I (GHR-I), insulin-like growth hormones land II (IGF-I and IGF-II, respectively), and insulin-like growth hormone binding protein-1b (IGFBP-1b); and ii) protein turnover: including the target of rapamycin protein (TOR), proteasome 20 delta (Prot 20D), cathepsin L, calpains 1 and 2 (Capn 1 and Capn 2, respectively), and calpastatin long and short isoforms (CAST-L and CAST-S, respectively) were measured for each dietary treatment. The transcript levels of GHR-I and IGF-I increased linearly with the increase of dietary Met content (P <0.01), reflecting overall growth performances. The apparent capacity for hepatic protein degradation (derived from the gene expression of TOR, Prot 20D, Capn 1, Capn 2, CAST-L and CAST-S) decreased with increasing dietary Met level in a relatively linear manner. Our results suggest that Met availability affects, directly or indirectly, the expression of genes involved in the GH/IGF axis response and protein turnover, which are centrally involved in the regulation of growth. (C) 2014 Elsevier Inc. All rights reserved.

AB - The effects of dietary level of methionine were investigated in juvenile rainbow trout (Oncorhynchus mykiss) fed five plant-based diets containing increasing content of crystalline methionine (Met), in a six week growth trial. Changes in the hepatic expression of genes related to i) the somatotropic axis: including the growth hormone receptor I (GHR-I), insulin-like growth hormones land II (IGF-I and IGF-II, respectively), and insulin-like growth hormone binding protein-1b (IGFBP-1b); and ii) protein turnover: including the target of rapamycin protein (TOR), proteasome 20 delta (Prot 20D), cathepsin L, calpains 1 and 2 (Capn 1 and Capn 2, respectively), and calpastatin long and short isoforms (CAST-L and CAST-S, respectively) were measured for each dietary treatment. The transcript levels of GHR-I and IGF-I increased linearly with the increase of dietary Met content (P <0.01), reflecting overall growth performances. The apparent capacity for hepatic protein degradation (derived from the gene expression of TOR, Prot 20D, Capn 1, Capn 2, CAST-L and CAST-S) decreased with increasing dietary Met level in a relatively linear manner. Our results suggest that Met availability affects, directly or indirectly, the expression of genes involved in the GH/IGF axis response and protein turnover, which are centrally involved in the regulation of growth. (C) 2014 Elsevier Inc. All rights reserved.

KW - BIOCHEMISTRY

KW - ZOOLOGY

KW - BREAM SPARUS-AURATA

KW - MESSENGER-RNA EXPRESSION

KW - FACTOR-BINDING PROTEIN-1

KW - AMINO-ACID-CONCENTRATIONS

KW - FACTOR-I

KW - DIFFERENTIAL EXPRESSION

KW - NITROGEN-UTILIZATION

KW - SOMATOTROPIC AXIS

KW - ATLANTIC SALMON

KW - MUSCLE GROWTH

KW - Fish

KW - Crystalline amino acid

KW - Somatotropic axis

KW - Proteolytic pathways

KW - TOR

U2 - 10.1016/j.cbpb.2014.11.009

DO - 10.1016/j.cbpb.2014.11.009

M3 - Journal article

VL - 181

SP - 33

EP - 41

JO - Comparative Biochemistry and Physiology - Part B: Biochemistry & Molecular Biology

JF - Comparative Biochemistry and Physiology - Part B: Biochemistry & Molecular Biology

SN - 1096-4959

ER -