Dietary low-dose sucrose modulation of IQ-induced genotoxicity in the colon and liver of Big Blue((TM)) rats

P. Moller; Max Hansen; H. Autrup; J. Bornholt; Ulla Birgitte Vogel; A. M. Molck; H. Wallin; L. O. Dragsted; L. Risom; H. E. Poulsen; S. Loft

doi:10.1016/S0027-5107(03)00058-7

Dietary low-dose sucrose modulation of IQ-induced genotoxicity in the colon and liver of Big Blue((TM)) rats

P. Moller, Max Hansen, H. Autrup, J. Bornholt, Ulla Birgitte Vogel, A. M. Molck, H. Wallin, L. O. Dragsted, L. Risom, H. E. Poulsen, S. Loft

National Food Institute

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Earlier studies have indicated that sucrose increases 2-amino-3-methylimidazo[4,5-f]quinoline (IQ)-induced aberrant crypt foci in the colon. In this study, we investigated the role of sucrose in IQ-induced genotoxicity of the colon mucosa and liver. Big Blue(TM) rats were fed with IQ (20 ppm in feed) and/or sucrose (3.45 or 6.85 wt.% in feed) for 3 weeks. IQ increased DNA strand breaks in the colon, whereas the mutation frequency was increased in the liver. The level of IQ-induced DNA adducts was elevated in both colon mucosa cells and liver. In the liver, high sucrose intake increased the level of DNA adducts above that of IQ and low sucrose intake. Oxidative DNA damage detected in terms of 7-hydro-8-oxo-2'-deoxyguanosine by HPLC-EC, or endonuclease HI or formamidopyrimidine DNA glycosylase sensitive sites were unaltered in the colon and liver. Expression of ERCC1 and OGG1 mRNA levels were unaffected by IQ or sucrose feeding. Biomarkers of oxidative stress, including Vitamin C, malondialdehyde and protein oxidations (gamma-glutamyl semialdehyde and 2-amino adipic semialdehyde) were unaltered in plasma and in liver. In conclusion, sucrose feeding increases IQ-induced genotoxicity in liver but not in colon, suggesting different mechanisms for sucrose and IQ in colon mutagenesis. (C) 2003 Elsevier Science B.V. All rights reserved.

Original language	English
Journal	Mutation research
Volume	527
Issue number	1-2
Pages (from-to)	91-97
ISSN	0027-5107
DOIs	https://doi.org/10.1016/S0027-5107(03)00058-7
Publication status	Published - 2003

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10.1016/S0027-5107(03)00058-7

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Moller, P., Hansen, M., Autrup, H., Bornholt, J., Vogel, U. B., Molck, A. M., Wallin, H., Dragsted, L. O., Risom, L., Poulsen, H. E., & Loft, S. (2003). Dietary low-dose sucrose modulation of IQ-induced genotoxicity in the colon and liver of Big Blue((TM)) rats. Mutation research, 527(1-2), 91-97. https://doi.org/10.1016/S0027-5107(03)00058-7

@article{4918e6cc10a94209b1cb3de2c35d06d3,

title = "Dietary low-dose sucrose modulation of IQ-induced genotoxicity in the colon and liver of Big Blue((TM)) rats",

abstract = "Earlier studies have indicated that sucrose increases 2-amino-3-methylimidazo[4,5-f]quinoline (IQ)-induced aberrant crypt foci in the colon. In this study, we investigated the role of sucrose in IQ-induced genotoxicity of the colon mucosa and liver. Big Blue(TM) rats were fed with IQ (20 ppm in feed) and/or sucrose (3.45 or 6.85 wt.% in feed) for 3 weeks. IQ increased DNA strand breaks in the colon, whereas the mutation frequency was increased in the liver. The level of IQ-induced DNA adducts was elevated in both colon mucosa cells and liver. In the liver, high sucrose intake increased the level of DNA adducts above that of IQ and low sucrose intake. Oxidative DNA damage detected in terms of 7-hydro-8-oxo-2'-deoxyguanosine by HPLC-EC, or endonuclease HI or formamidopyrimidine DNA glycosylase sensitive sites were unaltered in the colon and liver. Expression of ERCC1 and OGG1 mRNA levels were unaffected by IQ or sucrose feeding. Biomarkers of oxidative stress, including Vitamin C, malondialdehyde and protein oxidations (gamma-glutamyl semialdehyde and 2-amino adipic semialdehyde) were unaltered in plasma and in liver. In conclusion, sucrose feeding increases IQ-induced genotoxicity in liver but not in colon, suggesting different mechanisms for sucrose and IQ in colon mutagenesis. (C) 2003 Elsevier Science B.V. All rights reserved.",

author = "P. Moller and Max Hansen and H. Autrup and J. Bornholt and Vogel, {Ulla Birgitte} and Molck, {A. M.} and H. Wallin and Dragsted, {L. O.} and L. Risom and Poulsen, {H. E.} and S. Loft",

year = "2003",

doi = "10.1016/S0027-5107(03)00058-7",

language = "English",

volume = "527",

pages = "91--97",

journal = "Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis",

issn = "0027-5107",

publisher = "Elsevier",

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}

Dietary low-dose sucrose modulation of IQ-induced genotoxicity in the colon and liver of Big Blue((TM)) rats. / Moller, P.; Hansen, Max; Autrup, H.; Bornholt, J.; Vogel, Ulla Birgitte; Molck, A. M.; Wallin, H.; Dragsted, L. O.; Risom, L.; Poulsen, H. E.; Loft, S.

In: Mutation research, Vol. 527, No. 1-2, 2003, p. 91-97.

Research output: Contribution to journal › Journal article › Research › peer-review

TY - JOUR

T1 - Dietary low-dose sucrose modulation of IQ-induced genotoxicity in the colon and liver of Big Blue((TM)) rats

AU - Moller, P.

AU - Hansen, Max

AU - Autrup, H.

AU - Bornholt, J.

AU - Vogel, Ulla Birgitte

AU - Molck, A. M.

AU - Wallin, H.

AU - Dragsted, L. O.

AU - Risom, L.

AU - Poulsen, H. E.

AU - Loft, S.

PY - 2003

Y1 - 2003

N2 - Earlier studies have indicated that sucrose increases 2-amino-3-methylimidazo[4,5-f]quinoline (IQ)-induced aberrant crypt foci in the colon. In this study, we investigated the role of sucrose in IQ-induced genotoxicity of the colon mucosa and liver. Big Blue(TM) rats were fed with IQ (20 ppm in feed) and/or sucrose (3.45 or 6.85 wt.% in feed) for 3 weeks. IQ increased DNA strand breaks in the colon, whereas the mutation frequency was increased in the liver. The level of IQ-induced DNA adducts was elevated in both colon mucosa cells and liver. In the liver, high sucrose intake increased the level of DNA adducts above that of IQ and low sucrose intake. Oxidative DNA damage detected in terms of 7-hydro-8-oxo-2'-deoxyguanosine by HPLC-EC, or endonuclease HI or formamidopyrimidine DNA glycosylase sensitive sites were unaltered in the colon and liver. Expression of ERCC1 and OGG1 mRNA levels were unaffected by IQ or sucrose feeding. Biomarkers of oxidative stress, including Vitamin C, malondialdehyde and protein oxidations (gamma-glutamyl semialdehyde and 2-amino adipic semialdehyde) were unaltered in plasma and in liver. In conclusion, sucrose feeding increases IQ-induced genotoxicity in liver but not in colon, suggesting different mechanisms for sucrose and IQ in colon mutagenesis. (C) 2003 Elsevier Science B.V. All rights reserved.

AB - Earlier studies have indicated that sucrose increases 2-amino-3-methylimidazo[4,5-f]quinoline (IQ)-induced aberrant crypt foci in the colon. In this study, we investigated the role of sucrose in IQ-induced genotoxicity of the colon mucosa and liver. Big Blue(TM) rats were fed with IQ (20 ppm in feed) and/or sucrose (3.45 or 6.85 wt.% in feed) for 3 weeks. IQ increased DNA strand breaks in the colon, whereas the mutation frequency was increased in the liver. The level of IQ-induced DNA adducts was elevated in both colon mucosa cells and liver. In the liver, high sucrose intake increased the level of DNA adducts above that of IQ and low sucrose intake. Oxidative DNA damage detected in terms of 7-hydro-8-oxo-2'-deoxyguanosine by HPLC-EC, or endonuclease HI or formamidopyrimidine DNA glycosylase sensitive sites were unaltered in the colon and liver. Expression of ERCC1 and OGG1 mRNA levels were unaffected by IQ or sucrose feeding. Biomarkers of oxidative stress, including Vitamin C, malondialdehyde and protein oxidations (gamma-glutamyl semialdehyde and 2-amino adipic semialdehyde) were unaltered in plasma and in liver. In conclusion, sucrose feeding increases IQ-induced genotoxicity in liver but not in colon, suggesting different mechanisms for sucrose and IQ in colon mutagenesis. (C) 2003 Elsevier Science B.V. All rights reserved.

U2 - 10.1016/S0027-5107(03)00058-7

DO - 10.1016/S0027-5107(03)00058-7

M3 - Journal article

VL - 527

SP - 91

EP - 97

JO - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

JF - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

SN - 0027-5107

IS - 1-2

ER -