Diet type dictates the gut microbiota and the immune response against Yersinia ruckeri in rainbow trout (Oncorhynchus mykiss)

Hans-Christian Ingerslev, Mikael Lenz Strube, Louise von Gersdorff Jørgensen, Inger Dalsgaard, Mette Boye, Lone Madsen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    This study investigated the influence of the rainbow trout (Oncorhynchus mykiss) commensal intestinal microbiota in connection to an experimental Yersina ruckeri infection, the causative agent of enteric redmouth disease. One marine and one plant diet was administered to two different groups of rainbow trout. The plant-based diet gave rise to an intestinal microbiota dominated by the genera Streptococcus, Leuconostoc and Weissella from phylum Firmicutes whereas phylum Proteobacteria/Bacteroidetes/Actinobacteria dominated the community in the marine fed fish. In connection to the Y. ruckeri bath challenge there was no effect of the diet type on the cumulative survival, but the number of Y. ruckeri positive fish as measured by plate count and the number of fish with a 'high' number of reads belonging to genus Yersinia as measured by 16S rRNA next-generation sequencing was higher for marine diet fed fish. Furthermore, the two experimental groups of fish showed a differential immune response, where Y. ruckeri challenged marine fed fish had a higher transcription of IL-1β and MBL-2 relative to challenged plant diet fed fish. The data suggest that the plant diet gave rise to a prebiotic effect favouring the presence of bacterial taxons proving protective in connection to bath challenge by Y. ruckeri.
    Original languageEnglish
    JournalFish and Shellfish Immunology
    Volume40
    Issue number2
    Pages (from-to)624-633
    ISSN1050-4648
    DOIs
    Publication statusPublished - 2014

    Keywords

    • IMMUNE response in fishes
    • Gut microbiota
    • 16S rRNA
    • Immune gene transcription
    • Yersinia ruckeri
    • Lactobacillaceae

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