Diet type and challenge by Yersinia Ruckeri influence the intestinal microbiota in rainbow trout (Oncorhynchus Mykiss)

Hans-Christian Ingerslev, Inger Dalsgaard, L. von Gersdorff Jørgensen, Lone Madsen

    Research output: Contribution to conferenceConference abstract for conferenceResearchpeer-review

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    Abstract

    In warm-blooded animals such as humans and pigs the intestinal microbial composition is dependent on the type of ingested diet. It is known that it also influences the immune system and prevent colonization of pathogenic bacteria. The question is if the gut microbiota has the same impact in lower vertebrates such as fish?
    To examine this rainbow trout fry were fed two different diets of either a marine or vegetable origin. At a size of about four gram a subset of the fish was bath challenged by Yersinia ruckeri serotype O1 and intestines were then sampled 5 days post challenge from challenged fish (n = 40) and non-challenged control fish (n = 40). Subsequent metagenomic examination based on the 16S rDNA gene was then performed using the Illumina HiSeq 2000 platform. Obtained sequences were paired, sorted, filtered, checked for chimeras and finally mapped against the Greengene database using the open-source package Bion-meta. Analysis of the microbial communities when then made for the non-infected control fish and Yersinia ruckeri challenged fish.
    For the non-infected control fish, the results showed two distinctly different microbial patterns in the intestines dependent on the administered diet type. Fish fed a marine based diet overall had a significantly higher amount of the class β-proteobacteria, while phylum Firmicutes was significantly higher abundant in the intestines of vegetable fed fish. The genera within phylum Firmicutes present in significantly higher amounts in vegetable fed fish were Weissella, Leuconostoc and Streptococcus. Genus Aeromonas from the γ-proteobacteria class was also present in significantly higher amounts in the vegetable fed fish.
    The microbial community was different in the fish that were challenged by Yersinia ruckeri. Challenged fish clustered into two groups according to the load of Yersinia ruckeri specific reads in their intestine; the main part of challenged fish (n = 34) had a low amount of Yersinia specific reads (≤ 1.2 % of total amount of reads), while a minor group (n = 6) had a high load ranging between 13.9 and 23.2 % of all reads. These ‘high Yersinia level’ fish had a significantly lower amount of reads from the order Burkholderiales relative to the ‘low Yersinia level’ fish and non-infected control fish. Further, the ‘high Yersinia level’ fish further clustered separately when analyzing the bacterial community on a PCA plot.
    The immunological examinations were performed by RT-qPCR in order to measure the expression of selected immune genes. The results showed a similar expression pattern between the two diet groups of non-infected fish, but the response differed between the two diet groups in challenged fish. Overall, the results indicate that the gut microbiota in rainbow trout, like in warm-blooded animals, is highly plastic according to the type of diet and does also seem to be involved in the immunological response in connection to pathogenic challenge.
    Original languageEnglish
    Publication date2013
    Number of pages1
    Publication statusPublished - 2013
    Event3rd International Symposium Genomics in Aquaculture (GIA 2013) - Bodø, Norway
    Duration: 4 Sep 20136 Sep 2013
    http://gia2013.org/

    Conference

    Conference3rd International Symposium Genomics in Aquaculture (GIA 2013)
    CountryNorway
    CityBodø
    Period04/09/201306/09/2013
    Internet address

    Bibliographical note

    Oral presentation.

    Keywords

    • Microbiota
    • Diet
    • 16S rDNA
    • Rainbow trout
    • Yersinia ruckeri

    Cite this

    Ingerslev, H-C., Dalsgaard, I., Jørgensen, L. V. G., & Madsen, L. (2013). Diet type and challenge by Yersinia Ruckeri influence the intestinal microbiota in rainbow trout (Oncorhynchus Mykiss). Abstract from 3rd International Symposium Genomics in Aquaculture (GIA 2013), Bodø, Norway.
    Ingerslev, Hans-Christian ; Dalsgaard, Inger ; Jørgensen, L. von Gersdorff ; Madsen, Lone. / Diet type and challenge by Yersinia Ruckeri influence the intestinal microbiota in rainbow trout (Oncorhynchus Mykiss). Abstract from 3rd International Symposium Genomics in Aquaculture (GIA 2013), Bodø, Norway.1 p.
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    author = "Hans-Christian Ingerslev and Inger Dalsgaard and J{\o}rgensen, {L. von Gersdorff} and Lone Madsen",
    note = "Oral presentation.; 3rd International Symposium Genomics in Aquaculture (GIA 2013) ; Conference date: 04-09-2013 Through 06-09-2013",
    year = "2013",
    language = "English",
    url = "http://gia2013.org/",

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    Ingerslev, H-C, Dalsgaard, I, Jørgensen, LVG & Madsen, L 2013, 'Diet type and challenge by Yersinia Ruckeri influence the intestinal microbiota in rainbow trout (Oncorhynchus Mykiss)' 3rd International Symposium Genomics in Aquaculture (GIA 2013), Bodø, Norway, 04/09/2013 - 06/09/2013, .

    Diet type and challenge by Yersinia Ruckeri influence the intestinal microbiota in rainbow trout (Oncorhynchus Mykiss). / Ingerslev, Hans-Christian; Dalsgaard, Inger; Jørgensen, L. von Gersdorff; Madsen, Lone.

    2013. Abstract from 3rd International Symposium Genomics in Aquaculture (GIA 2013), Bodø, Norway.

    Research output: Contribution to conferenceConference abstract for conferenceResearchpeer-review

    TY - ABST

    T1 - Diet type and challenge by Yersinia Ruckeri influence the intestinal microbiota in rainbow trout (Oncorhynchus Mykiss)

    AU - Ingerslev, Hans-Christian

    AU - Dalsgaard, Inger

    AU - Jørgensen, L. von Gersdorff

    AU - Madsen, Lone

    N1 - Oral presentation.

    PY - 2013

    Y1 - 2013

    N2 - In warm-blooded animals such as humans and pigs the intestinal microbial composition is dependent on the type of ingested diet. It is known that it also influences the immune system and prevent colonization of pathogenic bacteria. The question is if the gut microbiota has the same impact in lower vertebrates such as fish? To examine this rainbow trout fry were fed two different diets of either a marine or vegetable origin. At a size of about four gram a subset of the fish was bath challenged by Yersinia ruckeri serotype O1 and intestines were then sampled 5 days post challenge from challenged fish (n = 40) and non-challenged control fish (n = 40). Subsequent metagenomic examination based on the 16S rDNA gene was then performed using the Illumina HiSeq 2000 platform. Obtained sequences were paired, sorted, filtered, checked for chimeras and finally mapped against the Greengene database using the open-source package Bion-meta. Analysis of the microbial communities when then made for the non-infected control fish and Yersinia ruckeri challenged fish. For the non-infected control fish, the results showed two distinctly different microbial patterns in the intestines dependent on the administered diet type. Fish fed a marine based diet overall had a significantly higher amount of the class β-proteobacteria, while phylum Firmicutes was significantly higher abundant in the intestines of vegetable fed fish. The genera within phylum Firmicutes present in significantly higher amounts in vegetable fed fish were Weissella, Leuconostoc and Streptococcus. Genus Aeromonas from the γ-proteobacteria class was also present in significantly higher amounts in the vegetable fed fish.The microbial community was different in the fish that were challenged by Yersinia ruckeri. Challenged fish clustered into two groups according to the load of Yersinia ruckeri specific reads in their intestine; the main part of challenged fish (n = 34) had a low amount of Yersinia specific reads (≤ 1.2 % of total amount of reads), while a minor group (n = 6) had a high load ranging between 13.9 and 23.2 % of all reads. These ‘high Yersinia level’ fish had a significantly lower amount of reads from the order Burkholderiales relative to the ‘low Yersinia level’ fish and non-infected control fish. Further, the ‘high Yersinia level’ fish further clustered separately when analyzing the bacterial community on a PCA plot. The immunological examinations were performed by RT-qPCR in order to measure the expression of selected immune genes. The results showed a similar expression pattern between the two diet groups of non-infected fish, but the response differed between the two diet groups in challenged fish. Overall, the results indicate that the gut microbiota in rainbow trout, like in warm-blooded animals, is highly plastic according to the type of diet and does also seem to be involved in the immunological response in connection to pathogenic challenge.

    AB - In warm-blooded animals such as humans and pigs the intestinal microbial composition is dependent on the type of ingested diet. It is known that it also influences the immune system and prevent colonization of pathogenic bacteria. The question is if the gut microbiota has the same impact in lower vertebrates such as fish? To examine this rainbow trout fry were fed two different diets of either a marine or vegetable origin. At a size of about four gram a subset of the fish was bath challenged by Yersinia ruckeri serotype O1 and intestines were then sampled 5 days post challenge from challenged fish (n = 40) and non-challenged control fish (n = 40). Subsequent metagenomic examination based on the 16S rDNA gene was then performed using the Illumina HiSeq 2000 platform. Obtained sequences were paired, sorted, filtered, checked for chimeras and finally mapped against the Greengene database using the open-source package Bion-meta. Analysis of the microbial communities when then made for the non-infected control fish and Yersinia ruckeri challenged fish. For the non-infected control fish, the results showed two distinctly different microbial patterns in the intestines dependent on the administered diet type. Fish fed a marine based diet overall had a significantly higher amount of the class β-proteobacteria, while phylum Firmicutes was significantly higher abundant in the intestines of vegetable fed fish. The genera within phylum Firmicutes present in significantly higher amounts in vegetable fed fish were Weissella, Leuconostoc and Streptococcus. Genus Aeromonas from the γ-proteobacteria class was also present in significantly higher amounts in the vegetable fed fish.The microbial community was different in the fish that were challenged by Yersinia ruckeri. Challenged fish clustered into two groups according to the load of Yersinia ruckeri specific reads in their intestine; the main part of challenged fish (n = 34) had a low amount of Yersinia specific reads (≤ 1.2 % of total amount of reads), while a minor group (n = 6) had a high load ranging between 13.9 and 23.2 % of all reads. These ‘high Yersinia level’ fish had a significantly lower amount of reads from the order Burkholderiales relative to the ‘low Yersinia level’ fish and non-infected control fish. Further, the ‘high Yersinia level’ fish further clustered separately when analyzing the bacterial community on a PCA plot. The immunological examinations were performed by RT-qPCR in order to measure the expression of selected immune genes. The results showed a similar expression pattern between the two diet groups of non-infected fish, but the response differed between the two diet groups in challenged fish. Overall, the results indicate that the gut microbiota in rainbow trout, like in warm-blooded animals, is highly plastic according to the type of diet and does also seem to be involved in the immunological response in connection to pathogenic challenge.

    KW - Microbiota

    KW - Diet

    KW - 16S rDNA

    KW - Rainbow trout

    KW - Yersinia ruckeri

    M3 - Conference abstract for conference

    ER -

    Ingerslev H-C, Dalsgaard I, Jørgensen LVG, Madsen L. Diet type and challenge by Yersinia Ruckeri influence the intestinal microbiota in rainbow trout (Oncorhynchus Mykiss). 2013. Abstract from 3rd International Symposium Genomics in Aquaculture (GIA 2013), Bodø, Norway.