Dielectrophoretic manipulation of human chromosomes in microfluidic channels: extracting chromosome dielectric properties

Casper Hyttel Clausen, Maria Dimaki, Sonia Buckley, Winnie Edith Svendsen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    An investigation of the dielectric properties of polyamine buffer prepared human chromosomes is presented in this paper. Chromosomes prepared in this buffer are only a few micrometers in size and shaped roughly like spherical discs. Dielectrophoresis was therefore chosen as the method of manipulation combined with a custom designed microfluidic system containing the required electrodes for dielectrophoresis experiments. Our results show that although this system is presently not able to distinguish between the different chromosomes, it can provide average data for the dielectric properties of human chromosomes in polyamine buffer. These can then be used to optimize system designs for further characterization and even sorting. The experimental data from the dielectrophoretic manipulation were combined with theoretical calculations to extract a range of values for the permittivity and conductivity of human polyamine buffer prepared chromosomes.
    Original languageEnglish
    JournalBioChip Journal
    Volume5
    Issue number1
    Pages (from-to)56-62
    ISSN1976-0280
    DOIs
    Publication statusPublished - 2011

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