Abstract
Until recently, little focus was given to the presence of diarrhetic shellfish poisoning ( DSP) toxin esters in seafood products. However, during the last few years, the occurrence of a high percentage of esters of the total amount of DSP toxins present in some seafood products has been observed. Samples of Danish surf clams ( Spisola spp.) and blue mussels ( Mytilus edulis) from 1999 - 2004 were analysed by liquid chromatography coupled with tandem mass spectrometry ( LC/ MS/ MS) for the presence of DSP toxin esters. The samples contained only okadaic acid and esters of okadaic acid. The level of total okadaic acid equivalents ranged from 224 to 2516 mu g kg (-1) in surf clams. The percentage of okadaic acid esters of the total okadaic acid equivalents ranged from 83 to 98%, mean 95%. The level of total okadaic acid equivalents ranged from 43 to 1631 mu g kg (-1) in blue mussels. The percentage of okadaic acid esters of the total okadaic acid equivalents ranged from 21 to 86%, mean 59%. The probability of a high percentage of okadaic acid esters seems to increase with higher amounts of total okadaic acid equivalents in the bivalves. The large prevalence of DSP toxin esters are of particular importance because of the increased use of chemical methods instead of mouse bioassay for the detection of DSP toxicity.
| Original language | English |
|---|---|
| Journal | Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment |
| Volume | 22 |
| Issue number | 8 |
| Pages (from-to) | 743-751 |
| ISSN | 0265-203X |
| Publication status | Published - 2005 |
Keywords
- blue mussel
- liquid chromatography coupled with tandem mass spectrometry detection ( LC/MS/MS)
- diarrhetic shellfish poisoning (DSP) toxins
- food safety
- extraction efficiency
- DSP toxin ester
- surf clam