Diagnostic value of meat juice in early detection of classical swine fever infection

Louise Lohse, Åse Uttenthal, Thomas Bruun Rasmussen, Jens Nielsen

    Research output: Contribution to journalJournal articleResearchpeer-review

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    Abstract

    To evaluate the diagnostic potential of meat juice for early detection of Classical swine fever virus (CSFV), meat juice and serum samples from pigs experimentally infected with different strains of CSFV were compared for virus load. From all samples, viral RNA was extracted by automated procedure before real-time reverse transcription polymerase chain reaction analysis was performed. Viral RNA was detected in meat juice, but at a lower level than in corresponding serum. Sensitivity was calculated to 91% and specificity to 97%. Disagreements between meat juice and serum results were found when samples originated from pigs infected with low virulence CSFV strains and/or when samples were collected within the first days after infection. In conclusion, while not the first choice for sample material for CSFV diagnosis, meat juice may constitute a useful alternative for herd-based studies or when blood and/or target organ material is not available. Strain virulence and time points for sample collection after infection are factors of importance for diagnostic success.
    Original languageEnglish
    JournalJournal of Veterinary Diagnostic Investigation
    Volume23
    Issue number5
    Pages (from-to)1005-1008
    ISSN1040-6387
    DOIs
    Publication statusPublished - 2011

    Keywords

    • Swine
    • Reverse transcription polymerase chain reaction
    • Meat juice
    • Classical swine fever virus

    Cite this

    Lohse, Louise ; Uttenthal, Åse ; Rasmussen, Thomas Bruun ; Nielsen, Jens. / Diagnostic value of meat juice in early detection of classical swine fever infection. In: Journal of Veterinary Diagnostic Investigation. 2011 ; Vol. 23, No. 5. pp. 1005-1008.
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    abstract = "To evaluate the diagnostic potential of meat juice for early detection of Classical swine fever virus (CSFV), meat juice and serum samples from pigs experimentally infected with different strains of CSFV were compared for virus load. From all samples, viral RNA was extracted by automated procedure before real-time reverse transcription polymerase chain reaction analysis was performed. Viral RNA was detected in meat juice, but at a lower level than in corresponding serum. Sensitivity was calculated to 91{\%} and specificity to 97{\%}. Disagreements between meat juice and serum results were found when samples originated from pigs infected with low virulence CSFV strains and/or when samples were collected within the first days after infection. In conclusion, while not the first choice for sample material for CSFV diagnosis, meat juice may constitute a useful alternative for herd-based studies or when blood and/or target organ material is not available. Strain virulence and time points for sample collection after infection are factors of importance for diagnostic success.",
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    author = "Louise Lohse and {\AA}se Uttenthal and Rasmussen, {Thomas Bruun} and Jens Nielsen",
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    Diagnostic value of meat juice in early detection of classical swine fever infection. / Lohse, Louise; Uttenthal, Åse; Rasmussen, Thomas Bruun; Nielsen, Jens.

    In: Journal of Veterinary Diagnostic Investigation, Vol. 23, No. 5, 2011, p. 1005-1008.

    Research output: Contribution to journalJournal articleResearchpeer-review

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    T1 - Diagnostic value of meat juice in early detection of classical swine fever infection

    AU - Lohse, Louise

    AU - Uttenthal, Åse

    AU - Rasmussen, Thomas Bruun

    AU - Nielsen, Jens

    PY - 2011

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    N2 - To evaluate the diagnostic potential of meat juice for early detection of Classical swine fever virus (CSFV), meat juice and serum samples from pigs experimentally infected with different strains of CSFV were compared for virus load. From all samples, viral RNA was extracted by automated procedure before real-time reverse transcription polymerase chain reaction analysis was performed. Viral RNA was detected in meat juice, but at a lower level than in corresponding serum. Sensitivity was calculated to 91% and specificity to 97%. Disagreements between meat juice and serum results were found when samples originated from pigs infected with low virulence CSFV strains and/or when samples were collected within the first days after infection. In conclusion, while not the first choice for sample material for CSFV diagnosis, meat juice may constitute a useful alternative for herd-based studies or when blood and/or target organ material is not available. Strain virulence and time points for sample collection after infection are factors of importance for diagnostic success.

    AB - To evaluate the diagnostic potential of meat juice for early detection of Classical swine fever virus (CSFV), meat juice and serum samples from pigs experimentally infected with different strains of CSFV were compared for virus load. From all samples, viral RNA was extracted by automated procedure before real-time reverse transcription polymerase chain reaction analysis was performed. Viral RNA was detected in meat juice, but at a lower level than in corresponding serum. Sensitivity was calculated to 91% and specificity to 97%. Disagreements between meat juice and serum results were found when samples originated from pigs infected with low virulence CSFV strains and/or when samples were collected within the first days after infection. In conclusion, while not the first choice for sample material for CSFV diagnosis, meat juice may constitute a useful alternative for herd-based studies or when blood and/or target organ material is not available. Strain virulence and time points for sample collection after infection are factors of importance for diagnostic success.

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    KW - Reverse transcription polymerase chain reaction

    KW - Meat juice

    KW - Classical swine fever virus

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